Mouse Interleukin 17A, IL-17A ELISA Kit E1143Mo 96T
Mouse Interleukin-17A (IL-17A) ELISA Kit (E1143Mo) is a high-sensitivity sandwich ELISA designed for quantitative detection of Mouse IL-17A in serum, plasma, cell culture supernatants, tissue homogenates, and biological fluids. With a detection range of 2–600 ng/L and sensitivity of 0.97 ng/L, this kit delivers reliable and reproducible results for inflammation, autoimmune, and immunology research applications. For research use only.
Key Features
- High sensitivity: 0.97 ng/L
- Detection range: 2–600 ng/L
- Sandwich ELISA format
- High specificity and reproducibility
- Suitable for multiple sample types
- 96-well microplate format
- Easy-to-follow protocol
Research use only
Description
Mouse Interleukin-17A (IL-17A) ELISA Kit – E1143Mo
The Mouse Interleukin-17A (IL-17A) ELISA Kit (E1143Mo) is a high-sensitivity sandwich enzyme-linked immunosorbent assay designed for the quantitative detection of Mouse IL-17A in biological samples. This kit provides accurate and reproducible results for immunology, inflammation, autoimmune disease, and cytokine research applications.
Interleukin-17A (IL-17A) is a pro-inflammatory cytokine secreted primarily by Th17 cells and plays a critical role in immune response regulation, inflammatory diseases, and host defense mechanisms. This ELISA kit enables precise measurement of IL-17A levels across multiple biological sample types.
Key Features
- High sensitivity: 0.97 ng/L
- Detection range: 2–600 ng/L
- Sandwich ELISA format
- High specificity and reproducibility
- Suitable for multiple sample types
- 96-well microplate format
- Easy-to-follow protocol
- Research use only
Product Specifications
| Parameter | Details |
| Product Name | Mouse Interleukin-17A (IL-17A) ELISA Kit |
| Catalog Number | E1143Mo |
| Detection Target | Mouse IL-17A |
| Detection Range | 2–600 ng/L |
| Sensitivity | 0.97 ng/L |
| Assay Type | Sandwich ELISA |
| Sample Types | Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate |
| Detection Method | Colorimetric (450 nm) |
| Kit Size | 48T / 96T |
| Storage | -20°C or 2-8°C |
Intended Use
This kit is designed for quantitative detection of Mouse IL-17A in:
- Serum
- Plasma
- Cell culture supernatants
- Tissue homogenates
- Ascites
- Biological fluids
Assay Principle
The Mouse IL-17A ELISA kit uses a sandwich ELISA method:
- Pre-coated anti-Mouse IL-17A antibodies on microplate wells
- Standards and samples added
- Biotinylated Mouse IL-17A antibody added
- Streptavidin-HRP added
- Substrate solution added for color development
- Stop solution added
- Absorbance measured at 450 nm
Kit Components
According to pages 1–2, the kit includes:
- Pre-coated ELISA plate
- Standard solution (800 ng/L)
- Standard diluent
- Biotinylated Mouse IL-17A antibody
- Streptavidin-HRP
- Substrate solution A
- Substrate solution B
- Stop solution
- Wash buffer (25x)
- Plate sealers
- User manual
Sample Types Supported
Compatible sample types include:
- Serum
- Plasma
- Cell culture supernatant
- Tissue homogenates
- Urine
- Ascites
- Cerebrospinal fluid
Performance Characteristics
Sensitivity
- 0.97 ng/L
Detection Range
- 2–600 ng/L
Precision
Intra-Assay Precision
| Sample | Mean | CV |
| Sample 1 | 108.1 | 5.0% |
| Sample 2 | 299.4 | 3.2% |
| Sample 3 | 92.7 | 6.5% |
Inter-Assay Precision
- CV < 10%
Applications
- Immunology research
- Inflammation studies
- Autoimmune disease research
- Cytokine analysis
- Infection research
- Cancer research
- Drug discovery
- Cell signaling research
Storage & Stability
- Store at -20°C for up to 1 year
- Store at 2-8°C for up to 6 months
- Use within 1 month after opening
- Avoid repeated freeze-thaw cycles
Advantages
- High sensitivity detection
- Wide dynamic range
- Excellent reproducibility
- High specificity
- Easy protocol
- Reliable performance
- Research-grade quality
Important Notes
- For research use only
- Not for diagnostic use
- Follow protocol strictly
- Run samples in duplicate for best results
