Description

Mouse Interferon Gamma (IFN-γ) ELISA Kit – E0056Mo

The Mouse Interferon Gamma (IFN-γ) ELISA Kit (E0056Mo) is a highly sensitive sandwich enzyme-linked immunosorbent assay designed for the quantitative detection of Mouse Interferon Gamma (IFN-γ) in biological samples. This kit provides reliable and reproducible results for immunology research, cytokine analysis, inflammation studies, and disease model investigations.

Interferon Gamma (IFN-γ) is a key cytokine involved in immune response regulation, inflammation, and host defense mechanisms. This ELISA kit allows precise quantification of IFN-γ levels across a variety of sample types for research applications.

Key Features

• High sensitivity: 2.43 ng/L
• Detection range: 5–1000 ng/L
• Sandwich ELISA format
• High specificity and accuracy
• Excellent reproducibility
• Suitable for multiple sample types
• 96-well microplate format
• Easy-to-follow protocol
• Research use only

Product Specifications

Parameter	Details
Product Name	Mouse Interferon Gamma (IFN-γ) ELISA Kit
Catalog Number	E0056Mo
Detection Target	Mouse IFN-γ
Detection Range	5–1000 ng/L
Sensitivity	2.43 ng/L
Assay Type	Sandwich ELISA
Sample Types	Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate
Assay Time	Approx. 90 minutes
Detection Method	Colorimetric (450 nm)
Kit Size	48T / 96T
Storage	-20°C or 2-8°C

Intended Use

The Mouse IFN-γ ELISA Kit is designed for quantitative detection of Mouse Interferon Gamma in:

• Serum
• Plasma
• Cell culture supernatants
• Tissue homogenates
• Ascites
• Other biological fluids

The kit is intended for research use only and not for diagnostic applications.

Assay Principle

1. Pre-coated anti-mouse IFN-γ antibodies on microplate wells
2. Samples and standards added to wells
3. Biotinylated Mouse IFN-γ antibody added
4. Streptavidin-HRP conjugate added
5. Substrate solution added to develop color
6. Stop solution added
7. Absorbance measured at 450 nm

The color intensity is proportional to Mouse IFN-γ concentration in the sample.

Kit Components

• Pre-coated ELISA plate
• Standard solution (1280 ng/L)
• Standard diluent
• Biotinylated Mouse IFN-γ antibody
• Streptavidin-HRP
• Substrate solution A
• Substrate solution B
• Stop solution
• Wash buffer (25x)
• Plate sealers
• User manual

Sample Types Supported

Compatible sample types include:

• Serum
• Plasma
• Cell culture supernatant
• Tissue homogenates
• Urine
• Ascites
• Cerebrospinal fluid

Performance Characteristics

Sensitivity

• 2.43 ng/L

Detection Range

• 5–1000 ng/L

Precision

Intra-Assay Precision

Sample	Mean	CV
Sample 1	321.1	2.8%
Sample 2	174.6	5.1%
Sample 3	131.4	4.6%

Inter-Assay Precision

• CV < 10%

Applications

• Immunology research
• Cytokine analysis
• Inflammation research
• Infection studies
• Autoimmune disease research
• Cancer research
• Drug discovery
• Cell signaling studies

Storage & Stability

• Store at -20°C for 1 year
• Store at 2-8°C for 6 months
• Use reagents within 1 month after opening
• Avoid repeated freeze-thaw cycles

Advantages

• High sensitivity detection
• Wide detection range
• Excellent reproducibility
• High specificity
• Easy protocol
• Reliable performance
• Research-grade quality

Important Notes

• For research use only
• Not for diagnostic use
• Follow protocol carefully
• Run samples in duplicate for best results