Description

Human MMP12 (Matrix Metalloproteinase-12) ELISA Kit – ELK2355

The Human Matrix Metalloproteinase-12 (MMP12) ELISA Kit (Cat. No. ELK2355) is a high-performance enzyme-linked immunosorbent assay designed for the quantitative determination of MMP12 in biological samples. MMP12, also known as macrophage elastase, is an important enzyme involved in extracellular matrix degradation, inflammatory processes, and tissue remodeling.

This ELISA kit utilizes a sandwich immunoassay format to detect Human MMP12 with high sensitivity and specificity. The assay enables researchers to accurately measure MMP12 concentrations across a wide dynamic range, supporting studies in immunology, inflammation, and disease pathogenesis.

Key Features

High Sensitivity Detection

The assay provides highly sensitive detection of Human MMP12 with a sensitivity of:

0.12 ng/mL

This allows researchers to detect even low concentrations of the target protein in biological samples.

Wide Quantification Range

The kit measures Human MMP12 concentrations across the range:

0.32 ng/mL – 20 ng/mL

This dynamic detection range ensures accurate analysis across different experimental conditions.

Excellent Specificity

The assay uses highly specific antibodies against Human MMP12 and demonstrates minimal cross-reactivity with related proteins, enabling reliable protein quantification.

High Precision and Reproducibility

The kit demonstrates strong performance characteristics:

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

These parameters ensure consistent results across experiments and assay runs.

Assay Principle

The Human MMP12 ELISA Kit is based on the sandwich enzyme immunoassay technique:

1. Microplate wells are pre-coated with antibodies specific to Human MMP12.
2. Standards and samples are added and bind to the immobilized antibodies.
3. A biotin-conjugated detection antibody specific to MMP12 is added.
4. Streptavidin-HRP conjugate binds to the antibody complex.
5. Addition of TMB substrate produces a color reaction proportional to the amount of MMP12 present.
6. The reaction is stopped and absorbance is measured at 450 nm using a microplate reader.

The intensity of the color signal correlates directly with the concentration of MMP12 in the sample and is determined using a standard calibration curve.

Compatible Sample Types

The kit is suitable for measuring MMP12 levels in various biological samples, including:

• Serum
• Plasma (EDTA or Heparin)
• Other biological fluids

Proper sample preparation and storage are recommended to maintain protein stability and ensure accurate assay results.

Kit Components

The Human MMP12 ELISA Kit includes all reagents required to perform the assay:

• Pre-Coated ELISA Microplate
• Lyophilized MMP12 Standard
• Biotinylated Detection Antibody
• Streptavidin-HRP Conjugate
• Standard/Sample Dilution Buffer
• Biotinylated Antibody Diluent
• HRP Diluent
• Wash Buffer (25× Concentrate)
• TMB Substrate Solution
• Stop Solution
• Plate Covers

All components are optimized and quality tested to ensure reliable experimental performance.

Applications

This ELISA kit is widely used in life science and biomedical research fields, including:

• Inflammation and immune response studies
• Lung disease and COPD research
• Tissue remodeling and extracellular matrix degradation studies
• Cancer biology research
• Biomarker discovery and drug development

Storage Conditions

To maintain reagent stability and assay performance:

• Short-term storage: 4°C (up to 6 months)
• Long-term storage: −20°C (up to 1 year)
• Avoid repeated freeze-thaw cycles.

Important Notice

For Research Use Only (RUO).
This kit is intended strictly for laboratory research and not for diagnostic or clinical use.