Description

Human MMP1 (Matrix Metalloproteinase-1) ELISA Kit – ELK2183

The Human Matrix Metalloproteinase-1 (MMP1) ELISA Kit (Cat. No. ELK2183) is a high-performance sandwich enzyme-linked immunosorbent assay developed for the quantitative determination of Human MMP1 in a wide range of biological samples. MMP1 is a collagenase enzyme involved in extracellular matrix remodeling, tissue repair, and inflammatory responses, making it an important biomarker in biomedical and molecular research.

This ELISA kit enables accurate measurement of MMP1 concentrations using a sensitive antibody-based detection system combined with colorimetric signal measurement. The assay is optimized for reproducibility and reliability, making it suitable for laboratory research in cell biology, disease mechanisms, and drug discovery.

Key Features

High Sensitivity Detection

The assay offers excellent analytical sensitivity, allowing detection of Human MMP1 at concentrations as low as:

0.062 ng/mL

This enables accurate measurement even in samples with low protein expression levels.

Wide Detection Range

The ELISA kit quantifies Human MMP1 across a working range of:

0.16 ng/mL – 10 ng/mL

This range supports reliable quantification across multiple biological matrices and experimental conditions.

High Specificity

The assay uses highly specific antibodies for Human MMP1 detection with minimal cross-reactivity or interference from related proteins, ensuring accurate measurement.

Reliable and Reproducible Performance

Performance validation demonstrates strong assay precision:

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

These parameters ensure consistent results across multiple experiments and assay runs.

Assay Principle

The Human MMP1 ELISA Kit utilizes a sandwich enzyme immunoassay technique for quantitative detection.

1. Microplate wells are pre-coated with an antibody specific to Human MMP1.
2. Standards and samples are added to the wells and bind to the immobilized antibody.
3. A biotinylated detection antibody specific for MMP1 is added.
4. Streptavidin-HRP conjugate binds to the biotinylated antibody.
5. Addition of TMB substrate generates a blue color proportional to the amount of MMP1 present.
6. The reaction is stopped, producing a yellow color measured at 450 nm using a microplate reader.

The intensity of the color signal is directly proportional to the concentration of MMP1 in the sample, which is determined by comparison with a standard curve.

Compatible Sample Types

The kit supports detection of Human MMP1 in a variety of biological samples, including:

• Serum
• Plasma
• Tissue homogenates
• Cell lysates
• Cell culture supernatants
• Cerebrospinal fluid (CSF)
• Other biological fluids

Proper sample preparation and storage conditions should be followed to maintain sample integrity and ensure accurate results.

Kit Components

Each kit contains all necessary reagents required to perform the assay:

• Pre-Coated ELISA Microplate
• Lyophilized MMP1 Standard
• Biotinylated Detection Antibody
• Streptavidin-HRP Conjugate
• Standard/Sample Dilution Buffer
• Biotinylated Antibody Diluent
• HRP Diluent
• Wash Buffer (25×)
• TMB Substrate Solution
• Stop Solution
• Plate Covers

All reagents are optimized and quality controlled to ensure accurate and reliable assay performance.

Applications

The Human MMP1 ELISA Kit is widely used in research fields including:

• Extracellular matrix degradation studies
• Cancer research and tumor metastasis analysis
• Inflammation and immune response studies
• Tissue remodeling and fibrosis research
• Drug discovery and biomarker validation

Storage Conditions

To maintain reagent stability and assay performance:

• Short-term storage: 4°C (up to 6 months)
• Long-term storage: −20°C (up to 1 year)
• Avoid repeated freeze-thaw cycles.

Important Notice

For Research Use Only (RUO).
This kit is intended for laboratory research applications and is not intended for diagnostic or clinical use.