Description

Mouse Tumor Necrosis Factor Alpha (TNF-A) ELISA Kit – E0117Mo

The Mouse Tumor Necrosis Factor Alpha (TNF-A) ELISA Kit (E0117Mo) is a highly sensitive sandwich enzyme-linked immunosorbent assay designed for the quantitative measurement of Mouse TNF-α in various biological samples. This kit provides excellent precision, wide detection range, and reproducible performance, making it suitable for cytokine research, inflammation studies, and immunology investigations.

Tumor Necrosis Factor Alpha (TNF-α) is a key cytokine involved in immune regulation, inflammation, apoptosis, and cell proliferation. Accurate detection of TNF-α is essential for studying immune responses, disease mechanisms, and therapeutic targets.

Intended Use

This sandwich ELISA kit is specifically designed for the accurate quantitative detection of Mouse Tumor Necrosis Factor Alpha (TNF-α) in:

• Serum
• Plasma
• Cell Culture Supernatants
• Tissue Homogenates
• Ascites
• Other Biological Fluids

The kit provides consistent and reliable results for research applications.

Assay Principle

This ELISA kit uses a sandwich immunoassay technique:

1. Pre-coated antibodies capture Mouse TNF-α in samples
2. Biotinylated detection antibody binds to captured antigen
3. Streptavidin-HRP conjugate binds to detection antibody
4. Substrate reaction produces measurable color
5. Absorbance measured at 450 nm for quantification

The color intensity is directly proportional to the concentration of Mouse TNF-α in the sample.

Technical Specifications

Parameter	Specification
Product Name	Mouse TNF-Alpha ELISA Kit
Catalog No.	E0117Mo
Assay Type	Sandwich ELISA
Sensitivity	2.49 ng/L
Detection Range	5–1000 ng/L
Sample Types	Serum, Plasma, Tissue, Cell Culture
Assay Time	Approx. 90–120 minutes
Detection Method	Colorimetric
Readout	450 nm
Storage	-20°C or 2-8°C
Format	96 Tests / 48 Tests

 

Kit Components (96 Tests)

• Pre-coated ELISA Plate
• Standard Solution
• Standard Diluent
• Streptavidin-HRP
• Substrate Solution A & B
• Stop Solution
• Wash Buffer Concentrate (25x)
• Biotinylated Mouse TNF-α Antibody
• Plate Sealer
• User Manual

Performance Characteristics

High Sensitivity

• Detection limit: 2.49 ng/L

Wide Detection Range

• 5–1000 ng/L

Precision

Intra-Assay Precision:

• CV range: 3.7% – 6.4%

Inter-Assay Precision:

• CV: <10%

Sample Types Supported

This ELISA kit supports multiple biological sample types:

• Serum
• Plasma
• Tissue Homogenates
• Cell Culture Supernatants
• Ascites
• Urine
• Cerebrospinal Fluid

This flexibility makes it ideal for broad research applications.

Applications

• Cytokine Research
• Immunology Studies
• Inflammation Research
• Cancer Research
• Autoimmune Disease Studies
• Drug Discovery
• Biomarker Research

Storage and Stability

• Store at -20°C for up to 1 year
• Store at 2-8°C for up to 6 months
• Use opened reagents within 1 month
• Avoid repeated freeze-thaw cycles

Advantages

• High sensitivity and specificity
• Wide detection range
• Excellent reproducibility
• Pre-coated microplate for convenience
• Fast and easy protocol
• Reliable performance
• Suitable for multiple sample types

Research Use

This product is intended for research use only and is not for diagnostic or therapeutic applications.

This Mouse TNF-Alpha ELISA Kit (E0117Mo) provides researchers with a reliable, sensitive, and efficient tool for accurate cytokine detection in mouse samples, making it an essential solution for immunology and inflammation research.