Description

Human VEGFA (Vascular Endothelial Growth Factor A) ELISA Kit – ELK1129

The Human VEGFA ELISA Kit (ELK1129) is a highly sensitive sandwich enzyme-linked immunosorbent assay designed for the quantitative detection of Human Vascular Endothelial Growth Factor A (VEGFA) in biological samples. This kit delivers reliable and reproducible performance for research applications related to angiogenesis, tumor biology, cardiovascular diseases, inflammation, and cell signaling studies.

VEGFA plays a crucial role in angiogenesis, vascular permeability, endothelial cell growth, and tissue repair, making it an essential biomarker in oncology, cardiovascular, and inflammatory research. This kit offers high specificity and sensitivity for precise VEGFA measurement in multiple sample types.

Key Features

• High sensitivity: 6.4 pg/mL
• Detection range: 15.63–1000 pg/mL
• Sandwich ELISA format
• High specificity with minimal cross-reactivity
• Excellent reproducibility
• Suitable for multiple sample types
• 96-well plate format
• Easy-to-follow protocol
• Research use only

Product Specifications

Parameter	Details
Product Name	Human VEGFA ELISA Kit
Catalog Number	ELK1129
Detection Target	Human VEGFA
Assay Type	Sandwich ELISA
Detection Range	15.63–1000 pg/mL
Sensitivity	6.4 pg/mL
Sample Types	Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants
Detection Method	Colorimetric (450 nm)
Kit Format	48T / 96T
Storage	4°C or -20°C

Intended Use

The Human VEGFA ELISA Kit is designed for quantitative determination of Human VEGFA in:

• Serum
• Plasma
• Tissue homogenates
• Cell lysates
• Cell culture supernatants
• Other biological fluids

This kit is intended for research use only and not for diagnostic applications.

Assay Principle

This kit uses the sandwich enzyme immunoassay method:

1. Microplate wells pre-coated with anti-human VEGFA antibodies
2. Standards and samples added to wells
3. Biotinylated detection antibody added
4. Streptavidin-HRP conjugate added
5. TMB substrate produces color signal
6. Stop solution added
7. Absorbance measured at 450 nm

The color intensity is directly proportional to VEGFA concentration in the sample.

Kit Components

The kit includes:

• Pre-coated microplate
• Lyophilized standard
• Biotinylated antibody
• Streptavidin-HRP
• Standard/sample diluent
• Antibody diluent
• HRP diluent
• Wash buffer (25×)
• TMB substrate solution
• Stop reagent
• Plate covers

Sample Types Supported

• Serum
• Plasma
• Tissue homogenates
• Cell lysates
• Urine
• Saliva
• Feces
• Cell culture supernatants
• Cerebrospinal fluid (CSF)

Performance Characteristics

Sensitivity

• 6.4 pg/mL

Detection Range

• 15.63–1000 pg/mL

Precision

• Intra-assay CV: <8%
• Inter-assay CV: <10%

This ensures consistent and reproducible results.

Recovery

Recovery performance:

Sample Type	Recovery Range	Average
Serum	78–96%	87%
EDTA Plasma	78–92%	85%
Heparin Plasma	80–95%	87%

Linearity

Linearity test results:

Sample Type	1:2	1:4	1:8	1:16
Serum	86-94%	85-95%	89-99%	85-95%
EDTA Plasma	89-97%	85-94%	79-96%	85-99%
Heparin Plasma	79-93%	89-97%	85-94%	87-101%

Applications

• Angiogenesis research
• Cancer research
• Cardiovascular research
• Inflammation studies
• Cell biology research
• Drug discovery
• Biomarker analysis
• Molecular biology research

Storage & Stability

• Store unopened kit at 4°C (short-term)
• Store at -20°C (long-term)
• Avoid repeated freeze-thaw cycles
• Use reagents within recommended shelf life

Advantages

• High sensitivity detection
• Wide detection range
• Excellent reproducibility
• High specificity
• Easy protocol
• Reliable results
• Research-grade quality

Important Notes

• For research use only
• Not for diagnostic use
• Follow protocol strictly
• Run samples in duplicate for best results