Human VEGF(Vascular Endothelial Cell Growth Factor) ELISA Kit EH0327
Human VEGF(Vascular Endothelial Cell Growth Factor) ELISA Kit EH0327 - Image 2
Human VEGF(Vascular Endothelial Cell Growth Factor) ELISA Kit EH0327 - Image 3
Human VEGF(Vascular Endothelial Cell Growth Factor) ELISA Kit EH0327 - Image 4

Human VEGF(Vascular Endothelial Cell Growth Factor) ELISA Kit EH0327

Human VEGF ELISA Kit (EH0327) is a high-sensitivity sandwich ELISA designed for quantitative detection of human Vascular Endothelial Growth Factor (VEGF) in serum, plasma, cell culture supernatants, tissue homogenates, and biological fluids. With a detection range of 31.25–2000 pg/ml and sensitivity of 18.75 pg/ml, this kit provides accurate and reproducible results for angiogenesis, cancer, and cardiovascular research applications. For research use only.

Key Features

  • High sensitivity: 18.75 pg/ml
  • Detection range: 31.25–2000 pg/ml
  • Sandwich ELISA format
  • High specificity with minimal cross-reactivity
  • Excellent reproducibility
  • Suitable for multiple sample types
  • 96-well plate format
  • Fast detection (approx. 4 hours)

Research use only

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Description

Human VEGF ELISA Kit (EH0327)

The Human VEGF ELISA Kit (EH0327) is a highly sensitive sandwich enzyme-linked immunosorbent assay designed for the quantitative determination of human Vascular Endothelial Growth Factor (VEGF) in biological samples. This kit provides reliable detection for research applications involving angiogenesis, tumor biology, cardiovascular diseases, inflammation, and neurological disorders.

VEGF plays a critical role in angiogenesis, vascular permeability regulation, and endothelial cell proliferation. It is widely used as a biomarker in cancer research, cardiovascular diseases, and inflammatory conditions. This ELISA kit delivers high sensitivity, specificity, and reproducibility for accurate measurement of VEGF concentrations.

Key Features

  • High sensitivity: 18.75 pg/ml
  • Detection range: 31.25–2000 pg/ml
  • Sandwich ELISA format
  • High specificity with minimal cross-reactivity
  • Excellent reproducibility
  • Suitable for multiple sample types
  • 96-well plate format
  • Fast detection (approx. 4 hours)
  • Research use only

Product Specifications

Parameter Details
Product Name Human VEGF ELISA Kit
Catalog Number EH0327
Detection Target Human VEGF
Assay Type Sandwich ELISA
Detection Range 31.25–2000 pg/ml
Sensitivity 18.75 pg/ml
Sample Types Serum, Plasma, Cell culture supernatant, Tissue lysate
Assay Time Approx. 4 hours
Detection Method Colorimetric (450 nm)
Storage 2–8°C
Kit Size 48T / 96T

Intended Use

This kit is designed for in vitro quantitative determination of human VEGF in:

  • Serum
  • Plasma
  • Cell culture supernatant
  • Tissue homogenates
  • Cell lysates
  • Other biological fluids

This kit is intended for research use only and not for diagnostic applications.

Background

Vascular Endothelial Growth Factor (VEGF) is a growth factor involved in angiogenesis and vascular permeability regulation. It promotes endothelial cell proliferation and migration while maintaining vascular networks. VEGF plays an essential role in:

  • Tumor growth and metastasis
  • Embryonic development
  • Cardiovascular diseases
  • Inflammation
  • Neurological disorders

Several VEGF subtypes exist including VEGF-A, VEGF-B, VEGF-C, VEGF-D, and VEGF-E, with VEGF-A being the most extensively studied.

Assay Principle

The Human VEGF ELISA Kit uses a sandwich ELISA method:

  1. Anti-VEGF antibody coated microplate
  2. Standards and samples added
  3. Biotin-labeled detection antibody added
  4. HRP-Streptavidin conjugate added
  5. TMB substrate produces color signal
  6. Stop solution added
  7. Absorbance measured at 450 nm

The color intensity is proportional to VEGF concentration in the sample.

Kit Components

The kit includes:

  • Pre-coated ELISA microplate
  • Lyophilized VEGF standard
  • Biotin-labeled antibody
  • HRP-Streptavidin conjugate
  • TMB substrate
  • Sample dilution buffer
  • Antibody dilution buffer
  • Stop solution
  • Wash buffer (25X)
  • Plate sealer
  • Product manual

Performance Characteristics

Sensitivity

  • Detection sensitivity: 18.75 pg/ml
  • Detection range: 31.25–2000 pg/ml

Precision

According to page 13, the assay demonstrates excellent reproducibility:

  • Intra-assay CV: 4.68% – 5.69%
  • Inter-assay CV: 5.72% – 6.01%

This ensures consistent and reliable results.

Recovery

Recovery performance results:

Sample Type Recovery Range Average
Serum 88-104% 98%
EDTA Plasma 85-102% 94%
Heparin Plasma 91-105% 100%

These results confirm assay accuracy.

Linearity

Dilution linearity performance:

Sample Type 1:2 1:4 1:8
Serum 86-105% 85-101% 88-104%
EDTA Plasma 86-100% 85-99% 94-100%
Heparin Plasma 80-92% 85-97% 83-100%

Applications

  • Angiogenesis research
  • Cancer research
  • Cardiovascular disease studies
  • Inflammation research
  • Neurological disease studies
  • Biomarker discovery
  • Drug development
  • Cytokine analysis

Storage & Stability

  • Store kit at 2-8°C
  • Do not freeze
  • Use before expiry date
  • Follow storage guidelines for opened components

Advantages

  • High sensitivity detection
  • Wide detection range
  • Excellent reproducibility
  • High specificity
  • Easy protocol
  • Reliable results
  • Research-grade quality

Important Notes

  • For research use only
  • Not for diagnostic use
  • Run samples in duplicate for best results
  • Follow protocol strictly

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