Description

Human Cystatin C (Cys-C) ELISA Kit – ELK11040

The Human Cystatin C (Cys-C) ELISA Kit (Cat. No. ELK11040) is a highly sensitive and reliable sandwich enzyme-linked immunosorbent assay developed for the quantitative detection of Human Cystatin C in biological samples. Cystatin C is a cysteine protease inhibitor widely studied in biomedical research for its role in renal function, inflammation, and metabolic regulation.

This ELISA kit provides a convenient and reproducible platform for detecting Cystatin C concentrations in research samples. Using high-affinity antibodies and optimized reagents, the assay delivers precise measurement across a broad concentration range, making it suitable for molecular biology, biomedical, and pharmaceutical research applications.

Key Features

High Analytical Sensitivity

The assay offers excellent detection sensitivity with a limit of:

2.47 ng/mL

This allows accurate detection of low concentrations of Cystatin C in biological samples.

Wide Detection Range

The ELISA kit supports quantitative analysis within the range:

3.13 ng/mL – 200 ng/mL

This broad detection range enables reliable measurement of Cystatin C levels across various experimental conditions.

High Specificity

The assay utilizes highly specific antibodies for Human Cystatin C, ensuring accurate detection with minimal cross-reactivity or interference from related proteins.

Reliable Reproducibility

The kit demonstrates strong performance consistency:

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

These parameters ensure reliable experimental outcomes across multiple assays.

Assay Principle

The Human Cys-C ELISA Kit is based on the sandwich enzyme immunoassay technique:

1. Microplate wells are pre-coated with antibodies specific to Human Cystatin C.
2. Standards or samples containing Cys-C are added and bind to the immobilized antibodies.
3. A biotinylated detection antibody specific to Cystatin C is added, forming an antibody-antigen complex.
4. Streptavidin-HRP conjugate binds to the biotinylated antibody.
5. Following addition of the TMB substrate, a color reaction develops.
6. The reaction is stopped and absorbance is measured at 450 nm.

The intensity of the color is directly proportional to the concentration of Cystatin C in the sample and is quantified using a standard curve.

Compatible Sample Types

This ELISA kit is validated for detecting Cystatin C in a variety of biological samples, including:

• Serum
• Plasma (EDTA or Heparin)
• Tissue Homogenates
• Cell Lysates
• Cell Culture Supernatants
• Cerebrospinal Fluid (CSF)
• Other Biological Fluids

Proper sample preparation and storage protocols should be followed to ensure optimal assay accuracy and reliability.

Kit Components

The Human Cys-C ELISA Kit includes all reagents necessary to perform the assay:

• Pre-Coated ELISA Microplate
• Lyophilized Cystatin C Standard
• Biotinylated Detection Antibody
• Streptavidin-HRP Conjugate
• Standard/Sample Diluent Buffer
• Biotinylated Antibody Diluent
• HRP Diluent
• Wash Buffer (25× Concentrate)
• TMB Substrate Solution
• Stop Reagent
• Plate Covers

All components are optimized and quality-tested to ensure reliable assay performance.

Applications

The Human Cystatin C ELISA Kit is widely used in research applications such as:

• Renal function and kidney disease research
• Biomarker discovery studies
• Inflammation and metabolic research
• Protein expression analysis
• Pharmacological and drug development studies

Storage Conditions

To maintain reagent stability and assay performance:

• Short-term storage: 4°C (up to 6 months)
• Long-term storage: −20°C (up to 1 year)
• Avoid repeated freeze–thaw cycles to preserve reagent integrity.

Important Notice

• For Research Use Only (RUO)
• Not intended for clinical or diagnostic use.