Description

Human Superoxide Dismutase (Cu-Zn), SOD1 ELISA Kit – E4502Hu

The Human Superoxide Dismutase (Cu-Zn), SOD1 ELISA Kit (E4502Hu) is a highly sensitive and specific enzyme-linked immunosorbent assay designed for quantitative detection of Human SOD1 in various biological samples. This kit utilizes a sandwich ELISA technique to ensure precise and reproducible measurement, making it ideal for oxidative stress research, antioxidant enzyme studies, and disease biomarker analysis.

This research-grade ELISA kit offers excellent sensitivity, high specificity, and consistent performance, ensuring accurate detection of SOD1 levels across a wide range of biological samples.

Key Features

• High sensitivity detection (0.24 ng/ml)
• Wide detection range (0.5-180 ng/ml)
• Sandwich ELISA format for high specificity
• Excellent reproducibility and precision
• Suitable for multiple biological sample types
• Research use only

Product Specifications

Parameter	Details
Product Name	Human Superoxide Dismutase (Cu-Zn), SOD1 ELISA Kit
Catalog Number	E4502Hu
Detection Method	Sandwich ELISA
Detection Range	0.5-180 ng/ml
Sensitivity	0.24 ng/ml
Assay Type	Quantitative
Sample Types	Serum, Plasma, Cell culture supernatant, Tissue homogenate
Assay Time	Approx. 90 minutes
Plate Type	96-well / 48-well
Storage	-20°C (1 year) / 2-8°C (6 months)

 

Intended Use

This ELISA kit is designed for the quantitative detection of Human Superoxide Dismutase (Cu-Zn) (SOD1) in:

• Serum
• Plasma
• Cell culture supernatants
• Tissue homogenates
• Ascites
• Other biological fluids

The kit is intended for research use only and not for diagnostic procedures.

Assay Principle

The Human SOD1 ELISA Kit is based on the sandwich ELISA method:

1. Microplate wells are pre-coated with Human SOD1 antibodies
2. Samples are added and SOD1 binds to coated antibodies
3. Biotinylated detection antibody is added
4. Streptavidin-HRP binds to detection antibody
5. Substrate solution produces color reaction
6. Absorbance measured at 450 nm

The intensity of the color is directly proportional to the concentration of Human SOD1 present in the sample.

Kit Components

The kit includes:

• Pre-coated ELISA Plate
• Standard Solution
• Standard Diluent
• Biotinylated SOD1 Antibody
• Streptavidin-HRP
• Substrate Solution A
• Substrate Solution B
• Stop Solution
• Wash Buffer (25x)
• Plate Sealers
• User Manual

Sample Types

Compatible sample types include:

• Serum
• Plasma
• Cell Culture Supernatant
• Tissue Homogenates
• Urine
• Ascites
• Cerebrospinal Fluid

Performance Characteristics

Sensitivity

• 0.24 ng/ml

Detection Range

• 0.5–180 ng/ml

Precision

Intra-Assay Precision

• CV < 6%

Inter-Assay Precision

• CV < 10%

This ensures high reproducibility and reliable experimental outcomes.

Storage and Stability

• Store at -20°C for up to 1 year
• Store at 2-8°C for up to 6 months
• Use reagents within 1 month after opening
• Avoid repeated freeze-thaw cycles

Applications

• Oxidative stress research
• Antioxidant enzyme studies
• Neurological disease research
• Inflammation research
• Biomarker discovery
• Cell biology studies
• Molecular biology research

Advantages

• High sensitivity and specificity
• Easy-to-follow protocol
• Reproducible results
• Fast assay time
• Wide sample compatibility
• Research-grade quality

Important Notes

• For research use only
• Not intended for diagnostic use
• Follow protocol strictly for best results

Avoid cross contamination