Description

The Human ADP (Adiponectin) ELISA Kit (Cat. No. ELK1233) is a high-performance sandwich enzyme-linked immunosorbent assay designed for the quantitative detection of human adiponectin (ADP) in biological samples. This assay provides a reliable and sensitive analytical platform for studying adiponectin levels associated with metabolic regulation, insulin sensitivity, inflammation, and cardiovascular research.

Developed using high-affinity antibodies and optimized assay conditions, the kit ensures excellent specificity, high sensitivity, and reproducible results in laboratory research applications. The assay employs a microplate pre-coated with a Human ADP-specific antibody that captures the target protein present in samples. Detection is achieved through a biotinylated antibody and Streptavidin-HRP enzyme conjugate, producing a measurable color reaction after substrate incubation. The optical density is measured at 450 nm, allowing accurate quantification through a standard curve.

Key Features

High Sensitivity Detection

The kit provides a detection sensitivity of 75 pg/mL, enabling precise measurement of low concentrations of adiponectin in biological samples.

Wide Quantification Range

The assay supports a reliable detection range of:

0.47 ng/mL – 30 ng/mL

This allows researchers to analyze both low and high expression levels of adiponectin.

High Specificity

The assay demonstrates excellent specificity for Human ADP (Adiponectin) with minimal cross-reactivity with related proteins, ensuring accurate results.

Reliable Reproducibility

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

These performance characteristics ensure consistency between experiments.

Assay Principle

This ELISA kit uses a sandwich enzyme immunoassay technique:

1. Microplate wells are pre-coated with antibodies specific to Human ADP.
2. Standards or samples are added and the target antigen binds to the immobilized antibody.
3. A biotinylated detection antibody binds to the captured antigen.
4. Streptavidin-HRP is added, forming an enzyme-linked detection complex.
5. Addition of TMB substrate produces a color change proportional to the amount of ADP present.
6. The reaction is stopped and absorbance is measured at 450 nm to determine concentration using a standard curve.

Sample Types

This kit is validated for use with:

• Serum
• Plasma

Proper sample collection and storage protocols should be followed to ensure accurate results and prevent degradation.

Kit Components

The ELISA kit contains all essential reagents required for assay performance, including:

• Pre-Coated ELISA Microplate
• Lyophilized ADP Standard
• Biotinylated Detection Antibody
• Streptavidin-HRP Conjugate
• Standard/Sample Dilution Buffer
• HRP Diluent
• Biotinylated Antibody Diluent
• Wash Buffer (25×)
• TMB Substrate Solution
• Stop Solution
• Plate Covers

These components are optimized and quality-tested to ensure reliable experimental performance.

Applications

The Human ADP ELISA Kit is widely used in biomedical and clinical research studies including:

• Metabolic disease research
• Obesity and insulin resistance studies
• Diabetes research
• Cardiovascular disease investigations
• Endocrinology studies
• Inflammation and immune regulation research

Storage Conditions

• Short-term storage: 4°C (up to 6 months)
• Long-term storage: −20°C (up to 1 year)
• Avoid repeated freeze-thaw cycles to maintain reagent integrity.

Important Notice

• For Research Use Only (RUO)

Not intended for diagnostic or therapeutic applications.