Description

Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit – E0082Hu

The Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit (E0082Hu) is a high-performance enzyme-linked immunosorbent assay designed for the quantitative detection of human TNF-α in biological samples. This sandwich ELISA kit provides high sensitivity, excellent reproducibility, and reliable performance for cytokine and inflammation research.

Tumor Necrosis Factor Alpha (TNF-α) is a key inflammatory cytokine involved in immune regulation, autoimmune diseases, cancer research, and inflammatory responses. This kit enables accurate measurement of TNF-α levels across various sample types for advanced research applications.

Key Features

• High sensitivity detection (1.52 ng/L)
• Wide detection range (3–900 ng/L)
• Sandwich ELISA format for enhanced specificity
• Excellent reproducibility and precision
• Suitable for multiple sample types
• Research use only
• Easy-to-follow protocol

Product Specifications

Parameter	Details
Product Name	Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit
Catalog Number	E0082Hu
Detection Method	Sandwich ELISA
Detection Range	3-900 ng/L
Sensitivity	1.52 ng/L
Assay Type	Quantitative
Sample Types	Serum, Plasma, Cell culture supernatant, Tissue homogenate
Assay Time	Approx. 90 minutes
Format	96-Well / 48-Well
Storage	-20°C or 2-8°C

Intended Use

This kit is designed for the accurate quantitative detection of Human TNF-α in:

• Serum
• Plasma
• Cell culture supernatants
• Tissue homogenates
• Ascites
• Other biological fluids

The kit is intended for research use only and not for diagnostic applications.

Assay Principle

The Human TNF-α ELISA Kit uses the sandwich ELISA technique:

1. Microplate wells are pre-coated with Human TNF-α antibodies
2. Samples containing TNF-α bind to the coated antibodies
3. Biotinylated detection antibody is added
4. Streptavidin-HRP conjugate binds to detection antibody
5. Substrate solution produces measurable color
6. Reaction stopped and absorbance read at 450 nm

The color intensity is directly proportional to TNF-α concentration in the sample.

Kit Components

The kit includes:

• Pre-coated ELISA plate
• Standard solution
• Standard diluent
• Biotinylated TNF-α antibody
• Streptavidin-HRP
• Substrate solution A
• Substrate solution B
• Stop solution
• Wash buffer (25x)
• Plate sealers
• User manual

Sample Types Supported

This kit is validated for:

• Serum
• Plasma
• Cell culture supernatants
• Tissue homogenates
• Urine
• Ascites
• Cerebrospinal fluid

Performance Characteristics

Sensitivity

• 1.52 ng/L

Detection Range

• 3-900 ng/L

Precision

Intra-Assay Precision

• CV < 5%

Inter-Assay Precision

• CV < 10%

This ensures consistent and reproducible results across experiments.

Storage and Stability

• Store at -20°C for up to 1 year
• Store at 2-8°C for up to 6 months
• Use reagents within 1 month after opening
• Avoid repeated freeze-thaw cycles

Applications

• Cytokine research
• Inflammation studies
• Immunology research
• Cancer research
• Autoimmune disease studies
• Biomarker discovery
• Cell biology research

Advantages

• High sensitivity and specificity
• Wide dynamic range
• Reliable reproducibility
• Easy protocol
• Fast assay time
• Research-grade quality

Important Notes

• For research use only
• Not intended for diagnostic use
• Follow protocol carefully
• Avoid cross-contamination