Description

Human Parkin ELISA Kit (ab212159)

The Human Parkin ELISA Kit (ab212159) is a high-performance SimpleStep Sandwich ELISA designed for rapid and accurate quantification of Human Parkin protein in cell culture extracts and tissue samples. This assay features a simplified single-wash 90-minute protocol, enabling efficient workflows without compromising sensitivity or accuracy.

The kit offers high sensitivity (9.7 pg/mL) and a broad detection range of 46.88–3000 pg/mL, making it suitable for a wide range of research applications including neurodegenerative disease research and mitochondrial function studies.

Key Features

• SimpleStep Sandwich ELISA technology
• Fast 90-minute assay
• Single wash protocol
• High sensitivity: 9.7 pg/mL
• Detection range: 46.88 – 3000 pg/mL
• Colorimetric detection at 450 nm
• 96-well plate format
• High reproducibility and precision
• Research use only

Intended Use

The Human Parkin ELISA Kit is designed for quantitative detection of Human Parkin protein in:

• Cell culture extracts
• Tissue extracts

Assay Principle

This kit utilizes SimpleStep Sandwich ELISA technology:

1. Samples and antibody cocktail are added simultaneously
2. Capture antibody binds to Parkin protein
3. Detector antibody forms antibody-analyte sandwich complex
4. Wash step removes unbound material
5. TMB substrate develops measurable color
6. Stop solution terminates reaction
7. Absorbance measured at 450 nm

This simplified approach significantly reduces assay time while maintaining high accuracy.

Kit Components

The Human Parkin ELISA Kit includes:

• Pre-coated 96-well microplate
• Capture antibody
• Detector antibody
• Recombinant Human Parkin standard
• Wash buffer
• TMB development solution
• Stop solution
• Sample diluent
• Antibody diluent
• Cell extraction buffer
• Plate seals

Performance Characteristics

Parameter	Specification
Assay Type	Sandwich ELISA
Detection Method	Colorimetric
Detection Range	46.88 – 3000 pg/mL
Sensitivity	9.7 pg/mL
Assay Time	90 minutes
Sample Type	Cell & Tissue Extracts
Format	96 Tests
Detection Wavelength	450 nm

Precision

The assay demonstrates excellent reproducibility:

• Intra-assay CV: 3%
• Inter-assay CV: 6%

Biological Background

Parkin is an E3 ubiquitin ligase protein involved in protein degradation and mitochondrial quality control. It plays an essential role in:

• Mitophagy regulation
• Protein degradation
• Cellular stress response
• Mitochondrial quality control

Mutations in Parkin are associated with Parkinson’s disease and neurodegenerative disorders, making this kit valuable for neuroscience research applications.

Applications

• Neurodegenerative disease research
• Parkinson’s disease research
• Mitochondrial function studies
• Autophagy research
• Cell biology research
• Protein degradation studies
• Biomedical research

Assay Workflow

1. Add samples and antibody cocktail
2. Incubate for 1 hour
3. Wash plate
4. Add TMB solution
5. Add stop solution
6. Read absorbance at 450 nm

This simplified workflow enables fast and reliable results in just 90 minutes.

Storage Conditions

• Shipped on blue ice
• Store at +4°C
• Do not freeze unless specified
• Follow storage instructions for best performance

Ordering Information

• Product Name: Human Parkin ELISA Kit
• Catalog Number: ab212159
• Assay Type: Sandwich ELISA
• Species: Human
• Format: 96 Tests
• Assay Time: 90 Minutes

Research Use Statement

This product is for research use only and not intended for diagnostic or therapeutic applications.