Description

Human PLGF (Placental Growth Factor) ELISA Kit – EH0022 (96T)

The Human PLGF ELISA Kit (EH0022) is a highly sensitive and specific sandwich enzyme-linked immunosorbent assay designed for the quantitative detection of Human Placental Growth Factor (PLGF) in various biological samples including serum, plasma, cell culture supernatant, and tissue lysates. This kit provides consistent and reproducible results for research applications in angiogenesis, cardiovascular disease, and pregnancy-related studies.

According to the Product Features table on page 2, the assay provides a detection range of 15.625–1000 pg/ml and sensitivity of 9.375 pg/ml, enabling accurate quantification even at low concentration levels.

Key Features

• High sensitivity: 9.375 pg/ml
• Detection range: 15.625–1000 pg/ml
• Sandwich ELISA method
• High specificity with minimal cross-reactivity
• Excellent reproducibility
• Suitable for multiple sample types
• 96-well plate format
• Reliable and consistent performance
• Research use only

Intended Use

This kit is designed for in vitro quantitative determination of Human PLGF in:

• Serum
• Plasma
• Cell culture supernatant
• Cell lysates
• Tissue homogenates
• Other biological samples

Assay Principle

According to the Principle of the Assay (Page 3), this kit uses sandwich ELISA technology:

1. Anti-PLGF antibodies are pre-coated on the microplate
2. Standards and samples are added
3. Biotin-labeled detection antibody binds PLGF
4. HRP-Streptavidin conjugate is added
5. TMB substrate develops color
6. Stop solution terminates reaction
7. Absorbance measured at 450 nm

The color intensity is proportional to the concentration of PLGF in the sample.

Kit Components

According to Kit Components table (Page 3), the kit includes:

• ELISA Microplate (96 wells)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate
• TMB Substrate
• Sample Dilution Buffer
• Antibody Dilution Buffer
• Wash Buffer (25X)
• Stop Solution
• Plate Sealers
• Instruction Manual

Performance Characteristics

Parameter	Specification
Assay Type	Sandwich ELISA
Detection Range	15.625–1000 pg/ml
Sensitivity	9.375 pg/ml
Detection Method	Colorimetric
Sample Types	Serum, Plasma, Cell Culture Supernatant
Assay Time	4 hours
Format	96 Tests
Detection Wavelength	450 nm

Precision & Accuracy

According to Precision data (Page 13):

• Intra-assay CV: ~5%
• Inter-assay CV: ~5%
• High reproducibility and reliability

Recovery performance:

• Serum: 91–104%
• EDTA Plasma: 89–101%
• Heparin Plasma: 87–103%

Linearity

The kit demonstrates excellent linearity across dilution ranges:

• 1:2 dilution: 88–101%
• 1:4 dilution: 85–98%
• 1:8 dilution: 85–103%

Applications

• Angiogenesis research
• Cardiovascular research
• Cancer research
• Pregnancy research
• Biomarker discovery
• Inflammatory disease research
• Molecular biology research
• Biomedical research

Assay Procedure Overview

According to Assay Procedure Summary (Page 9):

1. Add standards and samples
2. Incubate at 37°C
3. Add biotin-labeled antibody
4. Add HRP conjugate
5. Add TMB substrate
6. Add stop solution
7. Measure absorbance at 450 nm

Storage Conditions

• Store at 2–8°C
• Do not freeze
• Use before expiration date
• Store components properly after opening

Technical Specifications

• Species: Human
• Assay Type: Sandwich ELISA
• Detection Method: Colorimetric
• Kit Size: 96 Tests
• Sensitivity: 9.375 pg/ml
• Detection Range: 15.625–1000 pg/ml
• Sample Types: Serum, Plasma, Tissue, Cell Culture

Research Use Only

This product is intended for research use only and not for diagnostic or therapeutic procedures.