Description

Human NPT (Neopterin) ELISA Kit – ELK9065 (96 Tests)

The Human NPT (Neopterin) ELISA Kit (ELK9065) is a highly sensitive Competitive ELISA immunoassay designed for the quantitative detection of Human Neopterin (NPT) in a variety of biological samples. This kit provides reliable, reproducible, and accurate results suitable for research laboratories, academic institutions, and biotechnology applications.

Neopterin is widely used as a biomarker in immune activation studies, inflammatory response research, and clinical biomarker discovery, making this kit ideal for biomedical research applications.

Key Features

• Assay Type: Competitive Inhibition ELISA
• Detection Range: 0.16–10 ng/mL
• Sensitivity: 0.05 ng/mL
• High specificity with minimal cross-reactivity
• 96-well microplate format
• Excellent reproducibility
• Research use only

The assay demonstrates high specificity with no significant cross-reactivity or interference with related molecules.

Intended Use

This kit is designed for quantitative detection of Human Neopterin (NPT) in:

• Serum
• Plasma
• Cell lysates
• Cell culture supernatants
• Tissue homogenates
• Urine
• Saliva
• Other biological fluids

Assay Principle

The Human NPT ELISA Kit employs a Competitive Inhibition ELISA technique:

1. Microplate wells are pre-coated with Human NPT antigen
2. Samples and standards are added to wells
3. Biotinylated antibody binds to NPT
4. Streptavidin-HRP binds to antibody complex
5. TMB substrate produces measurable color
6. Stop solution terminates reaction
7. Absorbance measured at 450 nm

In this competitive assay, color intensity is inversely proportional to the concentration of Human NPT in the sample.

Kit Components

The kit includes:

• Pre-coated ELISA Microplate (96 wells)
• Lyophilized Standard
• Biotinylated Conjugate
• Streptavidin-HRP
• Standard/Sample Diluent
• Biotinylated Conjugate Diluent
• HRP Diluent
• Wash Buffer (25×)
• TMB Substrate Solution
• Stop Reagent
• Plate Covers

Performance Characteristics

Parameter	Specification
Assay Type	Competitive ELISA
Detection Range	0.16–10 ng/mL
Sensitivity	0.05 ng/mL
Sample Type	Serum, Plasma, Tissue
Assay Format	96 Wells
Detection Method	Colorimetric
Reading Wavelength	450 nm

Precision and Accuracy

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%
• High recovery across sample matrices
• Excellent linearity across dilution ranges

The recovery results show 82–109% recovery across serum and plasma samples, ensuring high assay reliability. (See performance data on page 12).

Applications

• Immunology research
• Inflammation studies
• Biomarker discovery
• Clinical research
• Pharmaceutical research
• Academic research
• Disease biomarker studies

Sample Types Supported

• Serum
• Plasma
• Tissue homogenates
• Cell lysates
• Cell culture supernatants
• Urine
• Saliva
• Other biological fluids

Storage Conditions

• Store unopened kit at 4°C or −20°C
• Store opened kit at 4°C
• Avoid repeated freeze-thaw cycles
• Use reagents within recommended storage period

Assay Summary

1. Add standards and samples
2. Add Biotinylated-Conjugate
3. Incubate at 37°C
4. Wash plate
5. Add Streptavidin-HRP
6. Add TMB substrate
7. Add stop solution
8. Measure absorbance at 450 nm

The step-by-step workflow diagram is shown on page 6 of the datasheet, illustrating incubation, washing, substrate reaction, and measurement stages.

Ordering Information

• Product Name: Human NPT (Neopterin) ELISA Kit
• Catalog Number: ELK9065
• Assay Type: Competitive ELISA
• Species: Human
• Format: 96 Tests

Research Use Statement

This product is for research use only and not intended for diagnostic or therapeutic applications.