Description

Free Testosterone ELISA Kit – DKO015

The Free Testosterone ELISA Kit (Cat. No. DKO015) is a competitive enzyme-linked immunosorbent assay designed for the quantitative measurement of free testosterone levels in human serum and plasma samples. Free testosterone represents the biologically active fraction of circulating testosterone and plays a critical role in endocrine regulation, reproductive health, and metabolic function.

This assay provides accurate and reliable quantification of free testosterone concentrations, making it suitable for research applications involving hormonal balance, reproductive studies, and endocrine function analysis.

Key Features

High Sensitivity Detection

The Free Testosterone ELISA Kit is designed to detect low concentrations of biologically active testosterone, enabling precise quantification across physiological ranges.

Competitive ELISA Format

This assay uses a competitive immunoassay format, ensuring:

• High specificity
• Reliable detection
• Accurate quantification
• Minimal cross-reactivity

Excellent Precision and Reproducibility

• High assay precision
• Reliable intra- and inter-assay reproducibility
• Consistent results across multiple experiments

Assay Principle

The Free Testosterone ELISA Kit employs a competitive enzyme immunoassay technique:

1. Microplate wells are coated with anti-testosterone antibodies
2. Standards and samples are added to the wells
3. Enzyme-labeled testosterone competes with free testosterone in the sample
4. Following incubation, unbound material is washed away
5. Substrate solution is added to generate color reaction
6. Reaction is stopped and absorbance measured at 450 nm

Because this is a competitive assay, color intensity is inversely proportional to the concentration of free testosterone in the sample.

Compatible Sample Types

This kit is validated for:

• Human Serum
• Human Plasma

Proper sample handling and storage are recommended to ensure accurate results.

Kit Components

The Free Testosterone ELISA Kit typically includes:

• Coated Microplate (96 wells)
• Testosterone Standards
• Enzyme Conjugate
• Sample Diluent
• Wash Buffer
• TMB Substrate Solution
• Stop Solution
• Plate Covers
• Instruction Manual

All reagents are optimized for accurate assay performance.

Applications

The Free Testosterone ELISA Kit is widely used in:

• Endocrinology research
• Reproductive biology studies
• Hormone imbalance research
• Metabolic and physiological research
• Clinical research applications (RUO only)

Advantages

• High sensitivity and specificity
• Easy-to-use protocol
• Rapid assay time
• Reliable reproducibility
• Minimal sample volume required

Storage Conditions

• Store kit at 2–8°C
• Do not freeze components unless specified
• Avoid repeated freeze–thaw cycles
• Protect substrate from light

Important Notice

For Research Use Only (RUO).
This product is intended for laboratory research purposes only and not for diagnostic or therapeutic use.