Human leucocyte antigen B27 ELISA Kit E0437Hu 48T
The Human Leucocyte Antigen B27 (HLA-B27) ELISA Kit (E0437Hu) is a high-sensitivity Sandwich ELISA assay designed for the quantitative detection of Human HLA-B27 in serum, plasma, tissue homogenates, and biological fluids. With a detection range of 0.5–300 ng/mL and sensitivity of 0.32 ng/mL, this kit ensures accurate and reliable results for research applications.
Key Features:
- Detection Range: 0.5–300 ng/mL
- Sensitivity: 0.32 ng/mL
- Assay Type: Sandwich ELISA
- Sample Type: Serum, Plasma, Tissue, Cell Culture
- Format: 48 Tests
- High specificity and reproducibility
- Research Use Only
Description
Human Leucocyte Antigen B27 (HLA-B27) ELISA Kit – E0437Hu (48 Tests)
The Human Leucocyte Antigen B27 (HLA-B27) ELISA Kit (E0437Hu) is a high-performance Sandwich ELISA immunoassay developed for the quantitative detection of Human HLA-B27 in multiple biological samples. This assay provides reliable, sensitive, and reproducible detection suitable for research laboratories and academic institutions.
Human leukocyte antigen B27 (HLA-B27) is widely used in immunology research, autoimmune disease studies, and biomarker investigations, making this kit ideal for biomedical and clinical research applications.
Key Features
- Detection range: 0.5–300 ng/mL
- High sensitivity: 0.32 ng/mL
- Sandwich ELISA method
- High specificity and reproducibility
- 48-well format
- Excellent assay precision
- Research use only
The assay demonstrates excellent precision with intra-assay CV <8% and inter-assay CV <10%, ensuring reliable experimental results.
Intended Use
This kit is designed for quantitative detection of Human HLA-B27 in:
- Serum
- Plasma
- Cell culture supernatants
- Tissue homogenates
- Ascites
- Urine
- Cerebrospinal fluid
- Other biological fluids
Assay Principle
This kit utilizes a Sandwich ELISA technique:
- Microplate wells are pre-coated with HLA-B27 antibodies
- Samples containing HLA-B27 bind to coated antibodies
- Biotinylated detection antibody is added
- Streptavidin-HRP binds to antibody complex
- Substrate solution develops measurable color
- Stop solution terminates reaction
- Absorbance measured at 450 nm
The intensity of color is proportional to the concentration of Human HLA-B27 in the sample.
Kit Components
The kit includes:
- Pre-coated ELISA plate (48 wells)
- Standard Solution
- Standard Diluent
- Biotinylated Human HLA-B27 Antibody
- Streptavidin-HRP
- Wash Buffer Concentrate (25×)
- Substrate Solution A
- Substrate Solution B
- Stop Solution
- Plate Sealer
- User Manual
Performance Characteristics
| Parameter | Specification |
| Assay Type | Sandwich ELISA |
| Detection Range | 0.5–300 ng/mL |
| Sensitivity | 0.32 ng/mL |
| Sample Type | Serum, Plasma, Tissue |
| Assay Format | 48 Wells |
| Detection Method | Colorimetric |
| Reading Wavelength | 450 nm |
Applications
- Immunology research
- Autoimmune disease studies
- Biomarker detection
- Clinical research
- Pharmaceutical research
- Academic research
- Inflammation studies
Sample Types Supported
- Serum
- Plasma
- Tissue homogenates
- Cell culture supernatants
- Urine
- Ascites
- Cerebrospinal fluid
- Other biological fluids
Storage Conditions
- Store at 2–8°C
- For long-term storage keep at −20°C
- Avoid repeated freeze-thaw cycles
- Use opened kit within 1 month for best performance
Assay Summary
- Add standards and samples
- Incubate for 60 minutes at 37°C
- Wash plate
- Add substrate solutions
- Add stop solution
- Measure absorbance at 450 nm
- Calculate results using standard curve
Ordering Information
- Product Name: Human Leucocyte Antigen B27 (HLA-B27) ELISA Kit
- Catalog Number: E0437Hu
- Assay Type: Sandwich ELISA
- Species: Human
- Format: 48 Tests
Research Use Statement
This product is for research use only and not intended for diagnostic or therapeutic applications.
