Description

Human Hyaluronic Acid (HA) ELISA Kit – ELK10906

The Human Hyaluronic Acid (HA) ELISA Kit (Cat. No. ELK10906) is a highly sensitive competitive inhibition enzyme-linked immunosorbent assay developed for the quantitative detection of Hyaluronic Acid in biological samples. Hyaluronic Acid is a key extracellular matrix component involved in tissue hydration, cell migration, and inflammation processes, making it an important biomarker in biomedical and pharmaceutical research.

This ELISA kit provides a robust and reproducible platform for detecting HA levels in serum and plasma samples. Using optimized reagents and high-specificity antibodies, the assay delivers reliable quantitative measurements across a wide concentration range, supporting diverse laboratory research applications.

Key Features

High Sensitivity Detection

The assay provides excellent analytical sensitivity with a detection limit of:

4.84 ng/mL

This enables precise quantification of Hyaluronic Acid even at low concentrations.

Wide Detection Range

The kit supports quantitative measurement within the range:

15.63 ng/mL – 1000 ng/mL

This broad range allows accurate measurement of HA levels across different sample types and experimental conditions.

Excellent Specificity

The assay demonstrates high specificity for Human Hyaluronic Acid with no significant cross-reactivity with related analogues, ensuring accurate and reliable detection.

Reproducible Results

The ELISA kit demonstrates strong reproducibility and reliability:

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

These performance characteristics ensure consistent results across multiple experiments.

Assay Principle

The Human HA ELISA Kit is based on the competitive inhibition enzyme immunoassay principle:

1. Microplate wells are pre-coated with Human Hyaluronic Acid.
2. Standards or samples are added together with a biotin-conjugated antibody specific for HA.
3. Streptavidin conjugated with Horseradish Peroxidase (HRP) binds to the biotinylated complex.
4. After addition of TMB substrate, a color reaction develops.
5. The reaction is stopped and absorbance is measured at 450 nm using a microplate reader.

Because this is a competitive assay, the signal intensity is inversely proportional to the concentration of HA in the sample. The HA concentration is determined using a standard calibration curve.

Compatible Sample Types

This ELISA kit supports detection of Hyaluronic Acid in various biological samples including:

• Serum
• Plasma (EDTA or Heparin)
• Urine
• Saliva
• Cell culture supernatants
• Other biological fluids

Proper sample handling and storage procedures should be followed to ensure optimal assay performance.

Kit Components

The Human HA ELISA Kit includes all necessary reagents to perform the assay:

• Pre-Coated ELISA Microplate
• Lyophilized HA Standard
• Biotinylated Conjugate
• Streptavidin-HRP
• Standard/Sample Diluent Buffer
• Biotinylated Conjugate Diluent
• HRP Diluent
• Wash Buffer (25×)
• TMB Substrate Solution
• Stop Reagent
• Plate Covers

All reagents are optimized and quality tested to ensure reliable experimental results.

Applications

The Human Hyaluronic Acid ELISA Kit is widely used in research areas including:

• Extracellular matrix biology
• Inflammation and tissue remodeling studies
• Liver fibrosis and metabolic research
• Cell biology and molecular signaling studies
• Drug discovery and biomarker research

Storage Conditions

To maintain optimal reagent stability:

• Short-term storage: 4°C (up to 6 months)
• Long-term storage: −20°C (up to 1 year)
• Avoid repeated freeze-thaw cycles.

Important Notice

• For Research Use Only (RUO)
• Not intended for diagnostic or clinical applications.