Description

Human MMP9 (Matrix Metalloproteinase-9) ELISA Kit – ELK10238

The Human Matrix Metalloproteinase-9 (MMP9) ELISA Kit (Cat. No. ELK10238) is a high-performance sandwich enzyme-linked immunosorbent assay designed for quantitative determination of MMP9 in biological samples. Matrix Metalloproteinase-9 is a key proteolytic enzyme involved in extracellular matrix remodeling, inflammatory responses, tumor progression, and tissue repair.

This ELISA kit provides a sensitive and reliable method for measuring MMP9 concentrations using a colorimetric detection system based on HRP-mediated enzymatic reaction. The assay is optimized for precision and reproducibility, making it suitable for a wide range of molecular biology and biomedical research applications.

Key Features

High Sensitivity Detection

The assay enables detection of Human MMP9 with a sensitivity of:

7.81 ng/mL

This allows reliable quantification of low-abundance protein levels in biological samples.

Wide Detection Range

The kit measures Human MMP9 concentrations across the range:

23.44 ng/mL – 1500 ng/mL

This wide dynamic range allows accurate analysis across multiple experimental conditions and sample types.

High Specificity

The assay uses highly specific antibodies targeting Human MMP9 with minimal cross-reactivity or interference with related proteins, ensuring precise quantification.

Reliable Reproducibility

The ELISA kit demonstrates strong assay performance:

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

These validation parameters ensure reliable results across different assay runs and laboratories.

Assay Principle

The Human MMP9 ELISA Kit utilizes the sandwich enzyme immunoassay technique:

1. Microplate wells are pre-coated with an antibody specific to Human MMP9.
2. Standards or samples are added to the wells and bind to the capture antibody.
3. A biotinylated detection antibody specific to MMP9 is added.
4. Streptavidin-HRP conjugate binds to the antibody complex.
5. Addition of TMB substrate produces a blue color that develops proportionally to the amount of MMP9 present.
6. The reaction is stopped and the absorbance is measured at 450 nm using a microplate reader.

The intensity of the color signal is directly proportional to the concentration of MMP9 in the sample and is calculated using a standard calibration curve.

Compatible Sample Types

This ELISA kit supports analysis of MMP9 in various biological samples including:

• Serum
• Plasma (EDTA or Heparin)
• Tissue homogenates
• Cell lysates
• Cell culture supernatants
• Cerebrospinal fluid (CSF)
• Other biological fluids

Proper sample preparation and storage procedures help maintain sample stability and ensure accurate assay performance.

Kit Components

The kit includes all essential reagents required for performing the assay:

• Pre-Coated ELISA Microplate
• Lyophilized MMP9 Standard
• Biotinylated Detection Antibody
• Streptavidin-HRP Conjugate
• Standard/Sample Dilution Buffer
• Biotinylated Antibody Diluent
• HRP Diluent
• Wash Buffer (25×)
• TMB Substrate Solution
• Stop Solution
• Plate Covers

All reagents are optimized and quality-tested to ensure reliable experimental performance.

Applications

The Human MMP9 ELISA Kit is widely used in research areas including:

• Cancer biology and tumor metastasis studies
• Inflammation and immune response research
• Tissue remodeling and fibrosis studies
• Cardiovascular disease research
• Drug discovery and biomarker validation

Storage Conditions

For optimal performance and reagent stability:

• Short-term storage: 4°C (up to 6 months)
• Long-term storage: −20°C (up to 1 year)
• Avoid repeated freeze-thaw cycles.

Important Notice

For Research Use Only (RUO).
This product is intended strictly for laboratory research and is not intended for diagnostic or clinical use.