Description

Rabbit TNFα (Tumor Necrosis Factor Alpha) ELISA Kit – ELK1164

The Rabbit TNFα (Tumor Necrosis Factor Alpha) ELISA Kit (Cat. No. ELK1164) is a highly sensitive immunoassay designed for the quantitative measurement of TNFα levels in rabbit biological samples. Tumor Necrosis Factor Alpha is a critical pro-inflammatory cytokine involved in immune regulation, inflammatory signaling pathways, and host defense mechanisms.

This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) technique, enabling precise detection of Rabbit TNFα across a wide concentration range. The assay is optimized for laboratory research investigating cytokine signaling, inflammation, immune responses, and disease models in rabbits.

Key Features

High Sensitivity Detection

The assay offers excellent analytical sensitivity with a minimum detectable concentration of:

1.22 pg/mL

This allows detection of very low levels of TNFα in biological samples.

Wide Detection Range

The kit quantifies Rabbit TNFα across the following dynamic range:

3.13 pg/mL – 200 pg/mL

This range supports detection across a variety of physiological and experimental conditions.

Excellent Specificity

The assay uses highly specific antibodies targeting Rabbit TNFα and demonstrates minimal cross-reactivity with related cytokines, ensuring reliable and accurate measurements.

High Precision and Reproducibility

The assay performance demonstrates strong reliability:

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

These parameters ensure consistent results across different experiments and assay runs.

Assay Principle

The Rabbit TNFα ELISA Kit utilizes the sandwich ELISA principle to detect TNFα in biological samples.

Assay Workflow:

1. Microplate wells are pre-coated with antibodies specific to Rabbit TNFα.
2. Standards and samples are added to the wells where TNFα binds to the immobilized antibodies.
3. A biotin-conjugated detection antibody specific for Rabbit TNFα is introduced.
4. Streptavidin-HRP enzyme conjugate binds to the antibody complex.
5. Addition of TMB substrate generates a colorimetric reaction proportional to the TNFα concentration.
6. The reaction is stopped and optical density is measured at 450 nm using a microplate reader.

The intensity of the color signal directly correlates with the concentration of Rabbit TNFα in the sample and is calculated using a standard curve.

The illustrated workflow diagram on page 6 visually demonstrates the sequential binding and enzymatic color development steps involved in the sandwich ELISA detection process.

Compatible Sample Types

The Rabbit TNFα ELISA Kit can detect TNFα in a wide range of biological samples including:

• Serum
• Plasma
• Tissue Homogenates
• Cell Lysates
• Cell Culture Supernatants
• Other Biological Fluids

Proper sample preparation and storage conditions should be followed to maintain protein integrity and ensure accurate quantification.

Kit Components

Each kit contains all reagents necessary for performing the ELISA assay:

• Pre-Coated ELISA Microplate
• Lyophilized Rabbit TNFα Standard
• Biotinylated Detection Antibody
• Streptavidin-HRP Conjugate
• Standard/Sample Dilution Buffer
• Biotinylated Antibody Diluent
• HRP Diluent
• Wash Buffer (25×)
• TMB Substrate Solution
• Stop Reagent
• Plate Covers

All components are optimized and quality-controlled to ensure reliable experimental performance.

Applications

The Rabbit TNFα ELISA Kit is widely used in biomedical and biological research including:

• Cytokine and inflammatory pathway studies
• Immunology and immune response research
• Infection and disease model research
• Drug discovery and pharmacological studies
• Experimental animal model research

Storage Conditions

To maintain optimal reagent stability and assay performance:

• Short-term storage: 4°C (up to 6 months)
• Long-term storage: −20°C (up to 1 year)
• Avoid repeated freeze-thaw cycles.

Important Notice

For Research Use Only (RUO).
This product is intended exclusively for laboratory research and not for diagnostic or therapeutic applications in humans or animals.