Description

E2 (Estradiol) ELISA Kit – ELK10219

The E2 (Estradiol) ELISA Kit (Cat. No. ELK10219) is a highly sensitive competitive enzyme-linked immunosorbent assay designed for the quantitative determination of Estradiol (E2) levels in biological samples. Estradiol is a major estrogen hormone that plays an essential role in reproductive biology, endocrine regulation, and metabolic processes.

This kit utilizes a competitive inhibition enzyme immunoassay technique, providing accurate and reproducible measurement of Estradiol levels across a broad detection range. The assay is optimized for research laboratories involved in endocrine studies, reproductive biology, hormonal regulation, and biomedical research.

Key Features

High Sensitivity Detection

The assay provides high analytical sensitivity with a minimum detectable concentration of:

14.45 pg/mL

This allows detection of low levels of Estradiol in biological samples.

Wide Detection Range

The kit quantifies Estradiol concentrations across the following range:

46.88 pg/mL – 3000 pg/mL

This wide dynamic range ensures accurate measurement across different experimental conditions.

High Specificity

The assay demonstrates excellent specificity with no significant cross-reactivity with Estradiol analogues, ensuring reliable results in complex biological samples.

Reliable Precision

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

These values ensure reproducible results across different runs and operators.

Assay Principle

The E2 ELISA Kit uses a competitive inhibition immunoassay method:

1. Microplate wells are pre-coated with Estradiol antigen
2. Standards or samples are added along with biotin-conjugated antibody
3. Estradiol in the sample competes with plate-bound antigen
4. Streptavidin-HRP conjugate binds to the complex
5. TMB substrate produces color reaction
6. Reaction is stopped and absorbance measured at 450 nm

Since this is a competitive assay, color intensity is inversely proportional to Estradiol concentration in the sample.

The workflow diagram on page 6 visually illustrates the binding, washing, and color development steps involved in the assay procedure.

Compatible Sample Types

This kit is validated for multiple biological samples including:

• Serum
• Plasma
• Tissue homogenates
• Cell lysates
• Cell culture supernatants
• Urine
• Saliva
• Other biological fluids

The sample preparation guidelines on pages 4–5 describe centrifugation, homogenization, and storage recommendations for optimal results.

Kit Components

The kit includes:

• Pre-coated ELISA Microplate
• Lyophilized Standard
• Biotinylated Conjugate
• Streptavidin-HRP
• Standard/Sample Diluent
• Wash Buffer (25×)
• TMB Substrate
• Stop Reagent
• Plate Covers

These reagents are optimized for high-performance Estradiol detection.

Assay Performance

Recovery Performance

• Serum: 83–95%
• EDTA Plasma: 88–102%
• Heparin Plasma: 79–97%

Linearity Performance

Serial dilution validation demonstrates reliable assay linearity across different sample concentrations.

These performance metrics confirm accuracy and reproducibility of the assay results.

Applications

The E2 (Estradiol) ELISA Kit is suitable for:

• Endocrinology research
• Reproductive biology studies
• Hormone regulation research
• Drug discovery and pharmacology
• Biomedical and clinical research (research use only)

Storage Conditions

• Store kit at 4°C (short-term)
• Store at −20°C (long-term)
• Avoid repeated freeze-thaw cycles
• Protect TMB substrate from light

These storage recommendations help maintain assay performance.

Important Notice

For Research Use Only (RUO).
This kit is intended strictly for laboratory research applications and not for diagnostic or therapeutic use.