Description

Human LEP (Leptin) ELISA Kit – ELK1160 (96 Tests)

The Human LEP (Leptin) ELISA Kit (ELK1160) is a highly sensitive Sandwich ELISA immunoassay designed for the quantitative detection of Human Leptin (LEP) in a variety of biological samples including serum, plasma, saliva, tissue homogenates, cell lysates, and cell culture supernatants. This assay provides accurate, reproducible, and reliable results for research applications.

Leptin is an important biomarker widely used in metabolism studies, obesity research, endocrine studies, and biomedical investigations, making this kit suitable for research laboratories, academic institutions, and biotechnology applications.

Key Features

• High sensitivity: 0.06 ng/mL
• Detection range: 0.16–10 ng/mL
• Sandwich ELISA method
• High specificity and minimal cross-reactivity
• Excellent reproducibility
• 96-well microplate format
• Research use only

The assay demonstrates excellent specificity for Human LEP with minimal interference from related molecules.

Intended Use

This kit is designed for quantitative detection of Human Leptin (LEP) in:

• Serum
• Plasma
• Saliva
• Tissue homogenates
• Cell lysates
• Cell culture supernatants
• Urine
• Cerebrospinal fluid
• Other biological fluids

Assay Principle

The Human LEP ELISA Kit uses a Sandwich ELISA technique:

1. Microplate wells are pre-coated with Human LEP antibody
2. Standards and samples are added to wells
3. Biotinylated detection antibody is added
4. Streptavidin-HRP binds to antibody complex
5. TMB substrate produces measurable color
6. Stop solution terminates reaction
7. Absorbance measured at 450 nm

The color intensity is proportional to the concentration of Human LEP present in the sample.

Kit Components

The kit includes:

• Pre-coated Microplate (96 wells)
• Standard (Lyophilized)
• Biotinylated Antibody
• Streptavidin-HRP
• Sample Diluent Buffer
• Biotinylated Antibody Diluent
• HRP Diluent
• Wash Buffer (25×)
• TMB Substrate Solution
• Stop Solution
• Plate Covers

Performance Characteristics

Parameter	Specification
Assay Type	Sandwich ELISA
Detection Range	0.16–10 ng/mL
Sensitivity	0.06 ng/mL
Sample Type	Serum, Plasma, Saliva, Tissue
Assay Format	96 Wells
Detection Method	Colorimetric
Reading Wavelength	450 nm

 

Precision and Accuracy

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%
• High recovery across serum and plasma samples
• Excellent linearity across dilution ranges

Applications

• Obesity research
• Metabolism studies
• Diabetes research
• Endocrine research
• Biomarker detection
• Clinical research
• Pharmaceutical research
• Academic research

Sample Types Supported

• Serum
• Plasma
• Saliva
• Tissue homogenates
• Cell lysates
• Cell culture supernatants
• Urine
• Cerebrospinal fluid
• Other biological fluids

Storage Conditions

• Store at 4°C for short-term use
• Store at -20°C for long-term storage
• Avoid repeated freeze-thaw cycles
• Follow storage instructions for best performance

Assay Summary

1. Add standards and samples
2. Incubate at 37°C
3. Add biotinylated antibody
4. Add Streptavidin-HRP
5. Add TMB substrate
6. Add stop solution
7. Measure absorbance at 450 nm
8. Calculate results using standard curve

Ordering Information

• Product Name: Human LEP (Leptin) ELISA Kit
• Catalog Number: ELK1160
• Assay Type: Sandwich ELISA
• Species: Human
• Format: 96 Tests

Research Use Statement

This product is for research use only and not intended for diagnostic or therapeutic applications.