Description

Human APOB (Apolipoprotein B) ELISA Kit – ELK1314

The Human APOB (Apolipoprotein B) ELISA Kit (Cat. No. ELK1314) is a high-performance sandwich enzyme immunoassay designed for the quantitative detection of human Apolipoprotein B (APOB) in multiple biological sample types. APOB is a critical structural component of lipoproteins involved in lipid metabolism and cardiovascular disease research, making this assay an essential tool for biomedical and clinical research laboratories.

This ELISA kit provides reliable, accurate, and reproducible measurement of APOB protein concentrations across a wide detection range. The assay utilizes a microplate pre-coated with antibodies specific to Human APOB, enabling efficient capture and detection of the target protein in experimental samples.

Key Features

High Sensitivity Detection

The kit provides a detection sensitivity of 0.073 µg/mL, enabling researchers to detect even low levels of APOB in biological samples.

Wide Detection Range

The assay supports quantitative detection within the range of:

0.16 µg/mL – 10 µg/mL

This broad dynamic range allows accurate analysis across different experimental conditions and sample concentrations.

High Specificity

The antibodies used in this assay demonstrate excellent specificity toward Human APOB, with minimal cross-reactivity with other proteins or analogues.

Excellent Reproducibility

Performance validation shows strong assay reliability:

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

These metrics ensure consistent results across experiments.

Assay Principle

The kit is based on a sandwich ELISA methodology:

1. Microplate wells are pre-coated with an antibody specific to Human APOB.
2. Standards and samples are added to the wells where APOB binds to the immobilized antibodies.
3. A biotinylated detection antibody specific for APOB is added.
4. Streptavidin-HRP conjugate binds to the detection antibody, forming an enzyme-linked complex.
5. Addition of TMB substrate generates a colorimetric reaction proportional to the amount of APOB present.
6. The reaction is stopped and absorbance is measured at 450 nm, allowing quantification using a standard curve.

Compatible Sample Types

This kit supports analysis of a variety of biological samples, including:

• Serum
• Plasma
• Tissue Homogenates
• Cell Lysates
• Cell Culture Supernatants
• Other Biological Fluids

Proper sample preparation and storage procedures should be followed to maintain sample integrity and assay accuracy.

Kit Components

The ELISA kit includes all essential reagents required for assay performance:

• Pre-Coated ELISA Microplate
• Lyophilized APOB Standard
• Biotinylated Detection Antibody
• Streptavidin-HRP Conjugate
• Standard/Sample Dilution Buffer
• Biotinylated Antibody Diluent
• HRP Diluent
• Wash Buffer (25×)
• TMB Substrate Solution
• Stop Solution
• Plate Covers

Each component is quality tested to ensure reliable experimental performance.

Applications

The Human APOB ELISA Kit is widely used in research fields including:

• Cardiovascular disease research
• Lipoprotein metabolism studies