Description

Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) ELISA Kit

The Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) ELISA Kit, Cat. No. MSI1145, is a high-sensitivity Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed for the quantitative detection of Human FABP3 in biological samples. FABP3 is a fatty acid-binding protein predominantly associated with muscle and heart tissues and is an important research biomarker for studies involving fatty acid metabolism and muscle- and cardiac-related biological processes.

This research-use ELISA kit provides a convenient and sensitive immunoassay format using a pre-coated microplate, a specific biotinylated antibody, Streptavidin-HRP, and a TMB substrate system. The assay generates a measurable colorimetric signal proportional to the amount of Human FABP3 present in the tested sample. Absorbance is measured at 450 nm, and sample concentrations are determined by comparison with the assay standard curve.

Key Product Features
Product: Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) ELISA Kit
Catalog No.: MSI1145
Assay Type: Sandwich ELISA
Target: Human FABP3
Sensitivity: 0.259 ng/mL
Detection Range: 0.63–40 ng/mL
Detectable Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
Detection Method: Colorimetric
Detection Wavelength: 450 ± 10 nm
Application: Research use only

The kit demonstrates high sensitivity and specificity for Human FABP3, with no significant cross-reactivity or interference reported between Human FABP3 and its analogues under the tested conditions.

Assay Principle

The Human FABP3 ELISA Kit is based on a Sandwich enzyme immunoassay principle. The microplate wells are pre-coated with an antibody specific to Human FABP3. Standards or samples are added to the wells, followed by a biotin-conjugated antibody specific to Human FABP3. Streptavidin conjugated with Horseradish Peroxidase (HRP) is subsequently added to bind the biotinylated antibody.

After addition of TMB substrate, wells containing Human FABP3 develop a measurable color. The enzymatic reaction is terminated using Stop Reagent, and the optical density is measured at 450 nm ± 10 nm. The concentration of Human FABP3 in unknown samples is calculated by comparing their absorbance values with the generated standard curve.

Sample Compatibility

The kit supports multiple biological sample matrices, including:

Serum
EDTA plasma
Heparin plasma
Tissue homogenates
Cell lysates
Cell culture supernatants
Urine
Saliva
Fecal samples
Cerebrospinal fluid (CSF)
Other biological fluids

Sample preparation requirements vary according to the sample type. Serum and plasma should be appropriately centrifuged before testing, while tissue and cell samples require suitable homogenization or lysis procedures. Samples should be aliquoted and stored appropriately to minimize degradation and repeated freeze-thaw cycles.

Detection Range and Standard Curve

The assay provides a detection range of 0.63–40 ng/mL. The standard series includes concentrations of 40, 20, 10, 5, 2.5, 1.25 and 0.63 ng/mL, with a 0 ng/mL blank. Samples falling outside the standard curve may require appropriate dilution and preliminary optimization.

Assay Workflow

The standard assay workflow consists of sample/standard addition, incubation, washing, addition of biotinylated antibody, incubation, addition of Streptavidin-HRP, further washing, TMB color development and termination of the reaction.

Typically, samples or standards are incubated at 37°C, followed by sequential antibody and enzyme-conjugate incubation steps. TMB substrate is developed in the dark, after which Stop Reagent is added and absorbance is read immediately at 450 nm using a compatible microplate reader.

Kit Components

The kit is available in 48T and 96T formats and contains the major reagents required for the ELISA assay, including:

Pre-coated Microplate
Lyophilized Standard
Biotinylated Antibody (100×)
Streptavidin-HRP (100×)
Standard/Sample Diluent Buffer
Biotinylated Antibody Diluent
HRP Diluent
25× Wash Buffer
TMB Substrate Solution
Stop Reagent
Plate Covers

The supplied reagent quantities vary according to the selected 48T or 96T configuration.

Performance Characteristics

The kit has demonstrated intra-assay precision of CV <8% and inter-assay precision of CV <10% under the conditions described in the product documentation. Reported recovery ranges were 80–94% for serum, 89–101% for EDTA plasma, and 82–95% for heparin plasma. Linearity testing was also performed using serially diluted serum, EDTA plasma and heparin plasma samples, with the reported results demonstrating acceptable agreement between calculated and expected concentrations across the tested dilution levels. Storage Conditions For unopened kits, short-term storage is specified at 4°C, while long-term storage is specified at -20°C. After opening, certain components including the pre-coated microplate, standard, biotinylated antibody and Streptavidin-HRP may require storage at -20°C, while other reagents are stored at 4°C according to the manufacturer's instructions. Repeated freeze-thaw cycles should be avoided. Required Equipment The following equipment and materials are required but are not supplied with the kit: Microplate reader capable of measuring absorbance at 450 ± 10 nm High-speed centrifuge Temperature-controlled incubator Precision single- or multi-channel pipettes Disposable pipette tips Double-distilled or deionized water Absorbent paper Research Applications The Human FABP3 ELISA Kit can be used in laboratory research involving Human FABP3 quantification, including studies of fatty acid-binding proteins, muscle and heart biology, protein expression, cellular and tissue research, and investigations using serum, plasma, tissue or cell-derived biological samples. Research Use Statement This product is intended for research use only (RUO) and is not intended for diagnostic use. The kit should be used strictly according to the supplied instructions, and reagents from different kits or batches should not be mixed unless specifically permitted by the product documentation. Human FABP3 ELISA Kit | Cat. No. MSI1145 offers a sensitive, specific and convenient sandwich ELISA platform for quantitative Human FABP3 research across a broad range of biological sample types.