Mouse SREBP2 (Sterol Regulatory Element-binding Protein 2) ELISA Kit
Mouse SREBP2 (Sterol Regulatory Element-binding Protein 2) ELISA Kit is a Sandwich ELISA designed for the quantitative detection of Mouse SREBP2 in tissue homogenates, cell lysates, and other biological fluids. The assay uses a specific capture antibody, biotinylated detection antibody, and Streptavidin-HRP for sensitive colorimetric detection.
Product Specifications:
Product: Mouse SREBP2 ELISA Kit
Cat. No.: MSI1000
Assay Type: Sandwich ELISA
Sample Types: Tissue homogenates, cell lysates & other biological fluids
Detection Range: 0.32–20 ng/mL
Sensitivity: 0.13 ng/mL
Detection: TMB colorimetric assay
Reading Wavelength: 450 nm
Application: Research Use Only (RUO)
The kit includes a pre-coated microplate, lyophilized standards, biotinylated antibody, Streptavidin-HRP, dilution buffers, wash buffer, TMB substrate, stop reagent, and plate covers.
Description
Special Explanation
1. *If the kit is opened, Store the whole kit at 4°C. If the kit is not used up in 1 week. Store the Pre
Coated Microplate, Standard ,Biotinylated Antibody and Streptavidin-HRP at -20°C, the rest reagents
at 4°C, please used up within 6 months.
*If the kit is not opened, store the whole kit: 4°C(short time storage, valid for 6 months); -20°C (long
term storage, valid for 1 year). Avoid repeated freeze-thaw cycles.
2. Do not use the kit beyond the expiration date.
3. If the whole kit is stored at -20°C, place the kit at 4°C the day before the experiment.
4. After opening the package, please check that all components are complete.
5. The cap must be tightened to prevent evaporation and microbial contamination. The reagents
volume is slightly more than the volume marked on labels, please use accurate measuring
equipment and do not pour directly into the vial.
All kit components have been formulated and quality control tested to function successfully. Do not mix or
substitute reagents or materials from other kits, detection effect of the kit will not be guaranteed if utilized
separately or substituted.
Materials Required, Not Supplied
1. Microplate reader capable of measuring absorbance at 450 ± 10 nm.
2. High-speed centrifuge.
3. Electro-heating standing-temperature cultivator.
4. Absorbent paper.
5. Double distilled water or deionized water.
6. Single or multi-channel pipettes with high precision and disposable tips.
7. Precision pipettes to deliver 2 μL to 1 mL volumes.
