Description

Rabbit IL6 (Interleukin-6) ELISA Kit – ELK2419

The Rabbit IL6 (Interleukin-6) ELISA Kit (Cat. No. ELK2419) is a highly sensitive immunoassay developed for the quantitative detection of Rabbit IL-6 in biological samples. Interleukin-6 is an important cytokine involved in immune regulation, inflammatory responses, cell signaling, and host defense mechanisms.

This ELISA kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique to accurately measure IL-6 concentrations across a broad dynamic range. The assay is optimized for research laboratories studying inflammatory pathways, immune responses, cytokine regulation, and experimental disease models in rabbits.

Key Features

High Sensitivity Detection

The assay provides highly sensitive measurement with a minimum detectable concentration of:

1.19 pg/mL

This sensitivity allows detection of very low concentrations of Rabbit IL-6 in biological samples.

Wide Quantification Range

The ELISA kit measures IL-6 concentrations across the following range:

3.13 pg/mL – 200 pg/mL

This dynamic range enables accurate detection across multiple experimental conditions.

Excellent Specificity

The assay uses highly specific antibodies targeting Rabbit IL-6, ensuring minimal cross-reactivity with related cytokines or analogues for accurate and reliable results.

Reliable Precision and Reproducibility

The kit demonstrates strong assay performance:

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

These parameters ensure consistent results across experiments and batches.

Assay Principle

The Rabbit IL6 ELISA Kit operates using a sandwich ELISA method, which provides high specificity and sensitivity.

Workflow Overview:

1. Microplate wells are pre-coated with antibodies specific to Rabbit IL-6.
2. Standards and samples are added, allowing IL-6 proteins to bind to the immobilized antibodies.
3. A biotin-conjugated detection antibody specific for Rabbit IL-6 is added.
4. Streptavidin-HRP enzyme conjugate binds to the antibody complex.
5. TMB substrate solution produces a color reaction proportional to IL-6 concentration.
6. The reaction is stopped and optical density is measured at 450 nm using a microplate reader.

The IL-6 concentration in samples is determined by comparing absorbance values with the standard calibration curve.

The assay workflow diagram on page 6 visually illustrates the sequential binding and enzymatic color development process used in this sandwich ELISA detection system.

Compatible Sample Types

The Rabbit IL6 ELISA Kit supports detection of IL-6 in various biological matrices including:

• Serum
• Plasma
• Tissue Homogenates
• Cell Lysates
• Cell Culture Supernatants
• Other Biological Fluids

Proper sample preparation and storage are recommended to preserve protein stability and ensure accurate results.

Kit Components

Each kit includes all reagents required to perform the assay:

• Pre-coated ELISA Microplate
• Lyophilized Rabbit IL-6 Standard
• Biotinylated Detection Antibody
• Streptavidin-HRP Conjugate
• Standard/Sample Dilution Buffer
• Biotinylated Antibody Diluent
• HRP Diluent
• Wash Buffer (25×)
• TMB Substrate Solution
• Stop Reagent
• Plate Covers

These components are optimized and quality-controlled to ensure consistent experimental performance.

Applications

The Rabbit IL6 ELISA Kit is widely used in biological and biomedical research including:

• Cytokine and inflammation research
• Immunology studies
• Infection and immune response research
• Pharmacological and drug development studies
• Animal model research involving inflammatory diseases

Storage Conditions

For optimal performance and reagent stability:

• Short-term storage: 4°C (up to 6 months)
• Long-term storage: −20°C (up to 1 year)
• Avoid repeated freeze-thaw cycles.

Important Notice

For Research Use Only (RUO).
This ELISA kit is intended strictly for laboratory research applications and not for diagnostic or therapeutic use in humans or animals.