Description

Rat FSH (Follicle Stimulating Hormone) ELISA Kit – ELK1315 (96T)

The Rat FSH (Follicle Stimulating Hormone) ELISA Kit – ELK1315 is a highly sensitive sandwich ELISA designed for quantitative measurement of Rat Follicle Stimulating Hormone (FSH) in biological samples. This kit provides high specificity, excellent reproducibility, and reliable performance for hormone analysis and endocrine research.

Follicle Stimulating Hormone (FSH) is an important reproductive hormone involved in ovarian follicle development and spermatogenesis. Accurate detection of FSH levels is essential for reproductive biology and hormonal regulation studies.

Product Overview

• Product Name: Rat FSH ELISA Kit
• Catalog Number: ELK1315
• Assay Type: Sandwich ELISA
• Format: 96 Tests
• Sensitivity: 0.72 mIU/mL
• Detection Range: 1.57–100 mIU/mL
• Detection Method: Colorimetric (450 nm)
• Research Use Only

Intended Use

This ELISA kit is designed for quantitative detection of Rat FSH in:

• Serum
• Plasma
• Cell culture supernatants
• Tissue homogenates
• Biological fluids

The kit provides reliable results for research applications in reproductive biology and hormone studies.

Assay Principle

This kit uses a Sandwich ELISA method:

1. Pre-coated antibody binds Rat FSH in samples
2. Biotinylated antibody binds captured antigen
3. Streptavidin-HRP conjugate binds to detection antibody
4. TMB substrate produces color reaction
5. Absorbance measured at 450 nm

The color intensity is proportional to Rat FSH concentration in the sample.

Kit Components (96 Tests)

The kit includes:

• Pre-coated Microplate (12 × 8 wells)
• Standard (Lyophilized)
• Biotinylated Antibody
• Streptavidin-HRP
• Standard/Sample Diluent
• Antibody Diluent
• HRP Diluent
• Wash Buffer (25×)
• TMB Substrate
• Stop Solution
• Plate Covers

Performance Characteristics

Sensitivity

• 0.72 mIU/mL

Detection Range

• 1.57–100 mIU/mL

Precision

• Intra-assay CV: <8%
• Inter-assay CV: <10%

Sample Types Supported

This kit supports multiple biological samples including:

• Serum
• Plasma
• Tissue homogenates
• Cell lysates
• Urine
• Saliva
• Feces
• Cell culture supernatants
• Cerebrospinal fluid

The sample collection guidelines on page 4–5 detail preparation methods for each sample type, ensuring accurate experimental results.

Assay Procedure Summary

1. Add standards and samples
2. Incubate at 37°C
3. Add biotinylated antibody
4. Add Streptavidin-HRP
5. Add TMB substrate
6. Stop reaction
7. Read absorbance at 450 nm

This streamlined workflow ensures consistent and reliable results.

Applications

• Reproductive biology research
• Hormone regulation studies
• Endocrinology research
• Fertility studies
• Biomedical research
• Drug development

Storage and Stability

• Store unopened kit at -20°C (1 year)
• Short-term storage at 4°C (6 months)
• Avoid repeated freeze-thaw cycles
• Store opened kit components properly for best performance

Advantages

• High sensitivity detection
• Wide dynamic range
• Excellent specificity
• Reliable reproducibility
• Easy-to-use protocol
• Multiple sample compatibility
• High-quality reagents

Product Specifications

Parameter	Details
Product Name	Rat FSH ELISA Kit
Catalog No	ELK1315
Assay Type	Sandwich ELISA
Sensitivity	0.72 mIU/mL
Detection Range	1.57–100 mIU/mL
Format	96 Tests
Detection	450 nm
Application	Hormone Detection

Research Use Statement

This kit is for research use only and is not intended for diagnostic applications.

The Rat FSH (Follicle Stimulating Hormone) ELISA Kit – ELK1315 provides researchers with a reliable and sensitive solution for hormone detection, making it an essential tool for reproductive biology, endocrinology, and biomedical research laboratories.