Description

Human KS (Keratan Sulfate) ELISA Kit – ELK0767 (96 Tests)

The Human KS (Keratan Sulfate) ELISA Kit (ELK0767) is a highly sensitive competitive inhibition enzyme immunoassay developed for the quantitative detection of Human Keratan Sulfate (KS) in multiple biological samples. This assay is optimized for research laboratories, academic institutions, and biotechnology applications requiring accurate measurement of KS levels.

This kit provides excellent sensitivity, specificity, and reproducibility, making it ideal for biomarker detection, extracellular matrix studies, and cartilage metabolism research.

Key Features

• Competitive inhibition ELISA method
• Detection range: 78.13–5000 pg/mL
• High sensitivity: 31.9 pg/mL
• High specificity with minimal cross-reactivity
• Excellent repeatability
• 96-well microplate format
• Research use only

The assay demonstrates high specificity for Human KS with no significant cross-reactivity with similar analogues.

Intended Use

This kit is designed for quantitative detection of Human Keratan Sulfate (KS) in:

• Serum
• Plasma
• Cell Lysates
• Tissue Homogenates
• Cell Culture Supernatants
• Urine
• Saliva
• Other Biological Fluids

Assay Principle

This assay employs a Competitive Inhibition ELISA technique:

1. Microplate wells are pre-coated with Human KS
2. Samples and standards are added
3. Biotinylated antibody binds to KS
4. Streptavidin-HRP binds to antibody complex
5. TMB substrate develops color
6. Reaction stopped with stop solution
7. Absorbance measured at 450 nm

The concentration of Human KS in samples is calculated using a standard curve, with inverse correlation between signal intensity and analyte concentration.

Kit Components

The kit includes:

• Pre-coated Microplate (96 wells)
• Lyophilized Standard
• Biotinylated-Conjugate
• Streptavidin-HRP
• Standard/Sample Diluent Buffer
• Biotinylated-Conjugate Diluent
• HRP Diluent
• Wash Buffer (25×)
• TMB Substrate Solution
• Stop Reagent
• Plate Covers

Performance Characteristics

Parameter	Specification
Assay Type	Competitive Inhibition ELISA
Detection Range	78.13–5000 pg/mL
Sensitivity	31.9 pg/mL
Sample Type	Serum, Plasma, Biological Fluids
Assay Format	96 Wells
Detection Method	Colorimetric
Reading Wavelength	450 nm

 

Applications

• Cartilage metabolism research
• Osteoarthritis studies
• Extracellular matrix research
• Biomarker detection
• Clinical research
• Drug discovery
• Academic research

Precision and Accuracy

• Intra-assay precision: CV <8%
• Inter-assay precision: CV <10%
• High recovery across serum and plasma samples
• Excellent assay linearity across dilution ranges

Sample Types Supported

• Serum
• Plasma
• Tissue homogenates
• Cell lysates
• Cell culture supernatants
• Urine
• Saliva
• Other biological fluids

Storage Conditions

• Store at 4°C for short-term use
• Store at -20°C for long-term storage
• Avoid repeated freeze-thaw cycles
• Use reagents according to recommended storage conditions

Assay Summary

1. Add standards and samples
2. Add biotinylated conjugate
3. Incubate at 37°C
4. Wash plate
5. Add Streptavidin-HRP
6. Add TMB substrate
7. Add stop solution
8. Read absorbance at 450 nm
9. Calculate results using standard curve

Ordering Information

• Product Name: Human KS (Keratan Sulfate) ELISA Kit
• Catalog Number: ELK0767
• Assay Type: Competitive Inhibition ELISA
• Species: Human
• Format: 96 Tests

Research Use Statement

This product is for research use only and not intended for diagnostic or therapeutic applications.