Description

Human ASGR1 (Asialoglycoprotein Receptor 1) ELISA Kit – ELK1676

The Human ASGR1 (Asialoglycoprotein Receptor 1) ELISA Kit (Cat. No. ELK1676) is a high-performance sandwich enzyme-linked immunosorbent assay developed for the quantitative measurement of Human ASGR1 protein levels in biological samples. ASGR1 is a membrane receptor primarily expressed in hepatocytes and plays an important role in glycoprotein clearance and liver function studies.

This ELISA kit provides a reliable and sensitive platform for detecting ASGR1 concentrations in research samples. Using highly specific antibodies and optimized reagents, the assay delivers reproducible results suitable for academic, biomedical, and pharmaceutical research laboratories.

Key Features

High Sensitivity Detection

The assay provides excellent sensitivity with a detection limit of 0.058 ng/mL, allowing detection of very low concentrations of ASGR1 protein in experimental samples.

Broad Detection Range

The kit supports accurate quantitative measurement across the range of:

0.16 ng/mL – 10 ng/mL

This dynamic range allows researchers to analyze samples with varying levels of ASGR1 expression.

High Specificity

The assay utilizes highly specific antibodies against Human ASGR1 to ensure accurate detection without significant cross-reactivity with related proteins.

Reliable Precision

Performance testing demonstrates strong reproducibility:

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

These parameters ensure consistent and reliable experimental results.

Assay Principle

The Human ASGR1 ELISA Kit uses a sandwich ELISA method to detect ASGR1 protein levels:

1. Microplate wells are pre-coated with antibodies specific to Human ASGR1.
2. Standards or samples containing ASGR1 are added and bind to the immobilized antibodies.
3. A biotinylated detection antibody specific for ASGR1 is introduced to form an antigen-antibody complex.
4. Streptavidin-HRP enzyme conjugate binds to the biotinylated antibody.
5. After addition of TMB substrate, a color reaction develops proportional to the amount of ASGR1 present.
6. The reaction is stopped and absorbance is measured at 450 nm, enabling quantitative analysis using a standard curve.

Compatible Sample Types

This ELISA kit is validated for detection of ASGR1 in:

• Tissue Homogenates
• Cell Lysates
• Cell Culture Supernatants
• Other Biological Fluids

Proper sample preparation and storage protocols should be followed to ensure optimal assay performance.

Kit Components

The kit contains all reagents necessary for performing the ELISA assay:

• Pre-Coated ELISA Microplate
• Lyophilized ASGR1 Standard
• Biotinylated Detection Antibody
• Streptavidin-HRP Conjugate
• Standard/Sample Dilution Buffer
• HRP Diluent
• Biotinylated Antibody Diluent
• Wash Buffer (25× Concentrate)
• TMB Substrate Solution
• Stop Solution
• Plate Covers

All reagents are optimized and quality-tested to ensure accurate and reproducible experimental results.

Applications

The Human ASGR1 ELISA Kit is widely used in biomedical and molecular biology research, including:

• Liver function and hepatocyte biology studies
• Glycoprotein metabolism research
• Receptor-mediated endocytosis studies
• Biomarker analysis in liver disease research
• Pharmaceutical and drug discovery research

Storage Conditions

• Short-term storage: 4°C (up to 6 months)
• Long-term storage: −20°C (up to 1 year)
• Avoid repeated freeze–thaw cycles to preserve reagent activity.

Important Notice

• For Research Use Only (RUO)
• Not intended for diagnostic or clinical use.