Description

Special Explanation

1. *If the kit is opened, Store the whole kit at 4°C. If the kit is not used up in 1 week. Store the Pre-Coated
Microplate, Standard ,Biotinylated-Conjugate and Streptavidin-HRP at -20°C, the rest reagents at 4°C,
please used up within 6 months.
*If the kit is not opened, store the whole kit: 4°C(short time storage, valid for 6 months); -20°C
(long-term storage, valid for 1 year). Avoid repeated freeze-thaw cycles
2. Do not use the kit beyond the expiration date.
3. If the whole kit is stored at -20°C, place the kit at 4°C the day before the experiment.
4. After opening the package, please check that all components are complete.
5. The cap must be tightened to prevent evaporation and microbial contamination. The reagents
volume is slightly more than the volume marked on labels, please use accurate measuring
equipment and do not pour directly into the vial.
All kit components have been formulated and quality control tested to function successfully. Do not mix
or substitute reagents or materials from other kits, detection effect of the kit will not be guaranteed if
utilized separately or substituted.

Materials Required, Not Supplied

1. Microplate reader capable of measuring absorbance at 450 ± 10 nm.
2. High-speed centrifuge.
3. Electro-heating standing-temperature cultivator.
4. Absorbent paper.
5. Double distilled water or deionized water.
6. Single or multi-channel pipettes with high precision and disposable tips.
7. Precision pipettes to deliver 2 μL to 1 mL volumes.

Safety Notes

1. This kit is only used for lab research and development and should not be used for human or animals.
2. Reagents should be regarded as hazardous substances and should be handled carefully and

3. Gloves, lab coats, and goggles should always be worn to avoid skin and eyes coming into contact
with Stop Reagent and TMB. In case of contact, wash thoroughly with water.

Test Principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate
provided in this kit has been pre-coated with Human IMA. Standards or samples are added to the
appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IMA. Next,
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After
TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of
450nm ± 10nm. The concentration of Human IMA in the samples is then determined by comparing the
OD of the samples to the standard curve.