Total antioxidant capacity(T-AOC) Assay Kit MSI1085
Determinate significance
There are many kinds of antioxidant macromolecules, small molecules and enzymes in the body,
which can eliminate all kinds of reactive oxygen species produced in the body, thus preventing
the generation of oxidative stress induced by reactive oxygen species.The total level of various
antioxidant macromolecules, small antioxidant molecules and enzymes in a system reflects the
total antioxidant capacity of the system.Therefore, it is of great significance to determine the
total antioxidant capacity in serum and other body fluids, tissues and cell lysate
Animals（Plants）Tissue：
The weight of the tissue was measured accurately, and the ratio of weight (g) : Volume (ml) 1:4,
adding 4 times volume of normal saline or PBS, mechanical homogenization under ice-water bath
condition, fully crushing the cells to release the antioxidants,centrifuged at 4℃, 12000rpm for 5
minutes, and take the supernatant to determination.
Calculate
Take the standard OD value as the horizontal coordinate and the standard OD concentration as
the vertical coordinate to make the Standard Curve.Get the curve formula by drawing software
(or excel table),Take the OD value measured by the sample into the calculation formula , and the
Description
2. Intended use
The kit is used for the determination of total antioxidant capacity in serum, plasma, tissue
homogenate, cells (or supernatants) , saliva and urine.
3. Assay principle
Under acidic conditions, the antioxidant can reduce Fe3+-TPTZZ to produce blue Fe2+-TPTZ. The
total antioxidant capacity can be calculated by reading the absorbance at 593 nm
4. Determinate significance
There are many kinds of antioxidant macromolecules, small molecules and enzymes in the body,
which can eliminate all kinds of reactive oxygen species produced in the body, thus preventing
the generation of oxidative stress induced by reactive oxygen species.The total level of various
antioxidant macromolecules, small antioxidant molecules and enzymes in a system reflects the
total antioxidant capacity of the system.Therefore, it is of great significance to determine the
total antioxidant capacity in serum and other body fluids, tissues and cell lysate
5. Applicable equipment
Various types of photometer or microsamples can be determined.
6. Sample preparation
1 、 Preparation of Serum, Saliva, urine, supernatant and other liquid samples：
Blood samples should be separated to get serum or plasma in time after collection. hemolysis
should be avoided during sampling. Direct determination of saliva, urine and supernatant of cells
Plasma is recommended for Heparin or Sodium Citrate anticoagulation and should not be treated
with EDTA.Literature report,The T-AOC of human serum was 0.5 ～2mM;The T-AOC of human
saliva was 0.3-1mM
2 、Animals（Plants）Tissue：
The weight of the tissue was measured accurately, and the ratio of weight (g) : Volume (ml) 1:4,
adding 4 times volume of normal saline or PBS, mechanical homogenization under ice-water bath
condition, fully crushing the cells to release the antioxidants,centrifuged at 4℃, 12000rpm for 5
minutes, and take the supernatant to determination.
3 、Cell:
The collection of not less than 1 million cells is recommended for cell scraping and should not be
digested with trypsin and EDTA. Add 200μLcold PBS, homogenate or ultrasound to fully crush the
cells and release their antioxidants,centrifuged at 4℃, 12000rpm for 5 minutes, and take the
supernatant to determination
