Description

Rat Testosterone (Testo) ELISA Kit – ELK10883 (96T)

The Rat Testosterone (Testo) ELISA Kit – ELK10883 is a high-performance competitive inhibition ELISA designed for quantitative measurement of Rat Testosterone levels in biological samples. This kit offers high sensitivity, excellent specificity, and reliable reproducibility for endocrine and reproductive research.

Testosterone is a key steroid hormone involved in reproductive development, metabolism, and physiological regulation. Accurate detection of testosterone levels is essential for hormone research and biomedical studies.

Product Overview

• Product Name: Rat Testosterone ELISA Kit
• Catalog Number: ELK10883
• Assay Type: Competitive Inhibition ELISA
• Format: 96 Tests
• Sensitivity: 0.64 ng/mL
• Detection Range: 1.56–100 ng/mL
• Detection Method: Colorimetric (450 nm)
• Research Use Only

Intended Use

This ELISA kit is designed for quantitative detection of Rat Testosterone in:

• Serum
• Plasma
• Tissue homogenates
• Cell lysates
• Urine
• Saliva
• Cell culture supernatants
• Other biological fluids

Assay Principle

The kit uses a Competitive Inhibition ELISA method:

1. Microplate pre-coated with Rat Testosterone antigen
2. Sample and biotinylated conjugate added simultaneously
3. Streptavidin-HRP added for detection
4. TMB substrate generates color reaction
5. Reaction stopped and read at 450 nm

In competitive ELISA, signal intensity is inversely proportional to testosterone concentration in the sample.

Kit Components (96 Tests)

The kit includes:

• Pre-coated Microplate (12 × 8 wells)
• Standard (Lyophilized)
• Biotinylated-Conjugate
• Streptavidin-HRP
• Standard/Sample Diluent
• Biotinylated-Conjugate Diluent
• HRP Diluent
• Wash Buffer (25×)
• TMB Substrate Solution
• Stop Reagent
• Plate Covers

Performance Characteristics

Sensitivity

• 0.64 ng/mL

Detection Range

• 1.56–100 ng/mL

Precision

• Intra-assay CV: <8%
• Inter-assay CV: <10%

Sample Types Supported

This kit supports multiple biological sample types:

• Serum
• Plasma
• Tissue homogenates
• Cell lysates
• Urine
• Saliva
• Cell culture supernatants
• Biological fluids

Assay Procedure Summary

1. Add standards and samples
2. Add biotinylated conjugate
3. Incubate at 37°C for 60 minutes
4. Add Streptavidin-HRP
5. Add TMB substrate
6. Add Stop Solution
7. Read absorbance at 450 nm

Performance Data

Recovery

• Serum: 82–95%
• EDTA Plasma: 80–95%
• Heparin Plasma: 86–99%

Linearity

• Excellent linearity across dilution series
• High accuracy across sample types
• Consistent performance across matrices

Applications

• Hormone research
• Endocrinology studies
• Reproductive biology
• Biomedical research
• Drug discovery
• Veterinary research
• Physiological studies

Storage and Stability

• Store unopened kit at -20°C (long-term)
• Store at 4°C for short-term use
• Avoid repeated freeze-thaw cycles
• Follow storage instructions for best performance

Advantages

• High sensitivity detection
• Competitive ELISA format
• Excellent specificity
• Wide detection range
• Reliable reproducibility
• Easy-to-use protocol
• Multiple sample compatibility

Product Specifications

Parameter	Details
Product Name	Rat Testosterone ELISA Kit
Catalog No	ELK10883
Assay Type	Competitive ELISA
Sensitivity	0.64 ng/mL
Detection Range	1.56–100 ng/mL
Format	96 Tests
Detection	450 nm
Application	Hormone Detection

Research Use Statement

This kit is for research use only and not intended for diagnostic use.

The Rat Testosterone (Testo) ELISA Kit – ELK10883 provides researchers with a reliable, sensitive, and efficient solution for hormone detection, making it an essential tool for endocrine, reproductive, and biomedical research laboratories.