Rat LH(Luteinizing Hormone) ELISA Kit ELK2367 96T
Rat LH (Luteinizing Hormone) ELISA Kit – ELK2367 (96T) is a high-sensitivity sandwich ELISA kit designed for quantitative detection of Rat LH in serum, plasma, and other biological fluids. This kit provides excellent specificity, high sensitivity, and reliable reproducibility for endocrine and reproductive research applications.
Key Features
- High Sensitivity: 0.17 mIU/mL
- Detection Range: 0.47–30 mIU/mL
- Assay Type: Sandwich ELISA
- Sample Types: Serum, Plasma, Biological Fluids
- High specificity with minimal cross-reactivity
- 96-well pre-coated microplate format
- Excellent precision (CV <10%)
- Colorimetric detection at 450 nm
- Research Use Only (RUO)
Description
Rat LH (Luteinizing Hormone) ELISA Kit – ELK2367 (96T)
The Rat LH (Luteinizing Hormone) ELISA Kit – ELK2367 is a high-sensitivity sandwich enzyme-linked immunosorbent assay designed for quantitative detection of Rat Luteinizing Hormone (LH) in biological samples. This kit offers excellent specificity, reproducibility, and sensitivity, making it ideal for reproductive biology, endocrine research, and hormone regulation studies.
Luteinizing Hormone (LH) plays a critical role in reproductive function, ovulation, and hormone regulation. Accurate measurement of LH levels is essential for endocrine and reproductive studies.
Product Overview
- Product Name: Rat LH ELISA Kit
- Catalog Number: ELK2367
- Assay Type: Sandwich ELISA
- Format: 96 Tests
- Sensitivity: 0.17 mIU/mL
- Detection Range: 0.47–30 mIU/mL
- Detection Method: Colorimetric (450 nm)
- Research Use Only
Intended Use
This ELISA kit is designed for quantitative detection of Rat LH in:
- Serum
- Plasma
- Tissue homogenates
- Cell lysates
- Urine
- Saliva
- Feces
- Cell culture supernatants
- Cerebrospinal fluid
Assay Principle
The kit uses a Sandwich ELISA technique:
- Pre-coated antibody binds Rat LH antigen
- Biotinylated antibody binds captured LH
- Streptavidin-HRP conjugate binds detection antibody
- TMB substrate produces measurable color
- Absorbance measured at 450 nm
The color intensity is proportional to Rat LH concentration.
Kit Components (96 Tests)
The kit includes:
- Pre-coated Microplate (12 × 8 wells)
- Standard (Lyophilized)
- Biotinylated Antibody
- Streptavidin-HRP
- Standard/Sample Diluent
- Antibody Diluent
- HRP Diluent
- Wash Buffer (25×)
- TMB Substrate Solution
- Stop Solution
- Plate Covers
Performance Characteristics
Sensitivity
- 0.17 mIU/mL
Detection Range
- 0.47–30 mIU/mL
Precision
- Intra-assay CV: <8%
- Inter-assay CV: <10%
Sample Types Supported
Supported biological samples include:
- Serum
- Plasma
- Tissue homogenates
- Cell lysates
- Urine
- Saliva
- Feces
- Cell culture supernatants
- Cerebrospinal fluid
Assay Procedure Summary
- Add standards and samples
- Incubate at 37°C for 80 minutes
- Add Biotinylated antibody
- Add Streptavidin-HRP
- Add TMB substrate
- Add Stop Solution
- Read absorbance at 450 nm
Applications
- Reproductive biology research
- Hormone regulation studies
- Endocrine research
- Fertility research
- Biomedical research
- Drug discovery
- Veterinary research
Performance Data
Recovery
- Serum: 87–99%
- EDTA Plasma: 78–92%
- Heparin Plasma: 85–99%
Linearity
- 1:2 to 1:16 dilution shows excellent linearity across sample types
- High accuracy across serum and plasma matrices
Storage and Stability
- Store unopened kit at -20°C (1 year)
- Store at 4°C (short-term storage)
- Avoid repeated freeze-thaw cycles
- Use reagents according to storage instructions
Advantages
- High sensitivity detection
- Excellent specificity
- Wide dynamic range
- High reproducibility
- Easy-to-use protocol
- Multiple sample compatibility
- Reliable performance
Product Specifications
| Parameter | Details |
| Product Name | Rat LH ELISA Kit |
| Catalog No | ELK2367 |
| Assay Type | Sandwich ELISA |
| Sensitivity | 0.17 mIU/mL |
| Detection Range | 0.47–30 mIU/mL |
| Format | 96 Tests |
| Detection | 450 nm |
| Application | Hormone Detection |
Research Use Statement
This product is for research use only and is not intended for diagnostic use.
The Rat LH (Luteinizing Hormone) ELISA Kit – ELK2367 provides researchers with a reliable, sensitive, and reproducible solution for hormone detection, making it ideal for reproductive biology, endocrinology, and biomedical research laboratories.
