Mouse Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit E0117Mo 96T
Mouse Tumor Necrosis Factor Alpha (TNF-A) ELISA Kit – E0117Mo (96 Tests) is a highly sensitive sandwich ELISA kit designed for accurate quantitative detection of Mouse TNF-α in serum, plasma, cell culture supernatants, tissue homogenates, ascites, and other biological fluids. With excellent sensitivity, reproducibility, and a wide detection range, this kit is ideal for immunology, inflammation, and cytokine research applications.
Key Features
- High Sensitivity: 2.49 ng/L
- Detection Range: 5–1000 ng/L
- Sample Types: Serum, Plasma, Tissue, Cell Culture, Ascites, Biological Fluids
- Assay Type: Sandwich ELISA
- Excellent Precision: Intra-assay CV <10%
- Pre-coated 96-well Microplate
- Fast and Reliable Results
- Storage: -20°C (1 year) or 2–8°C (6 months)
- Research Use Only (RUO)
Description
Mouse Tumor Necrosis Factor Alpha (TNF-A) ELISA Kit – E0117Mo
The Mouse Tumor Necrosis Factor Alpha (TNF-A) ELISA Kit (E0117Mo) is a highly sensitive sandwich enzyme-linked immunosorbent assay designed for the quantitative measurement of Mouse TNF-α in various biological samples. This kit provides excellent precision, wide detection range, and reproducible performance, making it suitable for cytokine research, inflammation studies, and immunology investigations.
Tumor Necrosis Factor Alpha (TNF-α) is a key cytokine involved in immune regulation, inflammation, apoptosis, and cell proliferation. Accurate detection of TNF-α is essential for studying immune responses, disease mechanisms, and therapeutic targets.
Intended Use
This sandwich ELISA kit is specifically designed for the accurate quantitative detection of Mouse Tumor Necrosis Factor Alpha (TNF-α) in:
- Serum
- Plasma
- Cell Culture Supernatants
- Tissue Homogenates
- Ascites
- Other Biological Fluids
The kit provides consistent and reliable results for research applications.
Assay Principle
This ELISA kit uses a sandwich immunoassay technique:
- Pre-coated antibodies capture Mouse TNF-α in samples
- Biotinylated detection antibody binds to captured antigen
- Streptavidin-HRP conjugate binds to detection antibody
- Substrate reaction produces measurable color
- Absorbance measured at 450 nm for quantification
The color intensity is directly proportional to the concentration of Mouse TNF-α in the sample.
Technical Specifications
| Parameter | Specification |
| Product Name | Mouse TNF-Alpha ELISA Kit |
| Catalog No. | E0117Mo |
| Assay Type | Sandwich ELISA |
| Sensitivity | 2.49 ng/L |
| Detection Range | 5–1000 ng/L |
| Sample Types | Serum, Plasma, Tissue, Cell Culture |
| Assay Time | Approx. 90–120 minutes |
| Detection Method | Colorimetric |
| Readout | 450 nm |
| Storage | -20°C or 2-8°C |
| Format | 96 Tests / 48 Tests |
Kit Components (96 Tests)
- Pre-coated ELISA Plate
- Standard Solution
- Standard Diluent
- Streptavidin-HRP
- Substrate Solution A & B
- Stop Solution
- Wash Buffer Concentrate (25x)
- Biotinylated Mouse TNF-α Antibody
- Plate Sealer
- User Manual
Performance Characteristics
High Sensitivity
- Detection limit: 2.49 ng/L
Wide Detection Range
- 5–1000 ng/L
Precision
Intra-Assay Precision:
- CV range: 3.7% – 6.4%
Inter-Assay Precision:
- CV: <10%
Sample Types Supported
This ELISA kit supports multiple biological sample types:
- Serum
- Plasma
- Tissue Homogenates
- Cell Culture Supernatants
- Ascites
- Urine
- Cerebrospinal Fluid
This flexibility makes it ideal for broad research applications.
Applications
- Cytokine Research
- Immunology Studies
- Inflammation Research
- Cancer Research
- Autoimmune Disease Studies
- Drug Discovery
- Biomarker Research
Storage and Stability
- Store at -20°C for up to 1 year
- Store at 2-8°C for up to 6 months
- Use opened reagents within 1 month
- Avoid repeated freeze-thaw cycles
Advantages
- High sensitivity and specificity
- Wide detection range
- Excellent reproducibility
- Pre-coated microplate for convenience
- Fast and easy protocol
- Reliable performance
- Suitable for multiple sample types
Research Use
This product is intended for research use only and is not for diagnostic or therapeutic applications.
This Mouse TNF-Alpha ELISA Kit (E0117Mo) provides researchers with a reliable, sensitive, and efficient tool for accurate cytokine detection in mouse samples, making it an essential solution for immunology and inflammation research.
