Description

Human VCAM-1 ELISA Kit (ELH-VCAM1)

The Human VCAM-1 ELISA Kit (ELH-VCAM1) from RayBiotech is a highly sensitive quantitative enzyme-linked immunosorbent assay designed for the detection of Human Vascular Cell Adhesion Molecule-1 (VCAM-1 / CD106) in biological samples. This kit utilizes a sandwich ELISA format to provide reliable and reproducible measurement of VCAM-1 levels in serum, plasma, and cell culture supernatants.

VCAM-1 is an important endothelial adhesion molecule involved in inflammation, immune response, cardiovascular diseases, and cancer progression. This kit provides accurate detection for advanced research applications in molecular biology and immunology.

Key Features

• High sensitivity detection (300 pg/ml)
• Quantitative sandwich ELISA format
• High specificity with minimal cross-reactivity
• Excellent reproducibility
• 96-well plate format
• Suitable for multiple sample types
• Easy-to-follow protocol
• Research use only

Product Specifications

Parameter	Details
Product Name	Human VCAM-1 ELISA Kit
Catalog Number	ELH-VCAM1
Assay Type	Sandwich ELISA
Detection Target	Human VCAM-1 / CD106
Sample Types	Serum, Plasma, Cell Culture Supernatant
Sensitivity	300 pg/ml
Plate Format	96-well
Detection Method	Colorimetric (450 nm)
Assay Time	Approx. 4–5 hours
Storage	-20°C (Long term), 4°C (Short term)

Intended Use

The Human VCAM-1 ELISA Kit is designed for quantitative measurement of human VCAM-1 in:

• Serum
• Plasma
• Cell culture supernatants

This kit is intended for research use only and not for diagnostic applications.

Assay Principle

The Human VCAM-1 ELISA Kit is based on sandwich ELISA technology:

1. Anti-human VCAM-1 antibody coated on microplate wells
2. Samples and standards added to wells
3. VCAM-1 binds to immobilized antibodies
4. Biotinylated detection antibody added
5. HRP-Streptavidin added
6. TMB substrate produces color signal
7. Stop solution added and absorbance measured at 450 nm

Color intensity is proportional to VCAM-1 concentration in the sample.

Kit Components

The kit includes:

• Human VCAM-1 coated microplate
• VCAM-1 standard protein
• Detection antibody
• HRP-Streptavidin
• TMB substrate reagent
• Stop solution
• Wash buffer (20X)
• Assay diluent A
• Assay diluent B (5X)
• Plate sealing film

Performance Characteristics

Sensitivity

• Minimum detectable dose: 300 pg/ml

According to page 5, the sensitivity was defined as the analyte concentration resulting in absorbance two standard deviations above the blank.

Spiking & Recovery

Mean recovery results (page 5):

Sample Type	Average Recovery	Range
Serum	94.42%	83–103%
Plasma	93.58%	82–102%
Cell Culture Media	98.76%	85–104%

These results demonstrate excellent assay accuracy.

Linearity

As shown in page 6, dilution linearity demonstrated:

• Serum: 93–94% recovery
• Plasma: 93–95% recovery
• Cell culture media: 94–96% recovery

This confirms consistent performance across sample dilutions.

Reproducibility

• Intra-Assay CV: <10%
• Inter-Assay CV: <12%

This ensures reliable and reproducible results.

Specificity

The kit shows no cross-reactivity with multiple cytokines and proteins including:

• Angiopoietin-1
• CD40
• IL-18
• ICAM-2
• MMP family proteins
• PDGF-AB
• TIMP-3
• And many others

This ensures highly specific detection of VCAM-1.

Applications

• Inflammation research
• Cardiovascular disease studies
• Immunology research
• Endothelial biology
• Cancer research
• Biomarker discovery
• Cytokine research

Storage & Stability

• Store kit at -20°C for up to 1 year
• Store at 4°C for up to 6 months
• Avoid repeated freeze-thaw cycles
• For extended storage: -80°C recommended

Advantages

• High sensitivity
• Excellent reproducibility
• High specificity
• Easy protocol
• Reliable performance
• Research-grade quality

Important Notes

• For research use only
• Not for diagnostic use
• Follow protocol strictly
• Run samples in duplicate for best results