Description

Human sCD163 (Soluble CD163) ELISA Kit – ELK8938 (96T)

The Human sCD163 ELISA Kit (ELK8938) is a high-performance sandwich enzyme-linked immunosorbent assay designed for quantitative detection of Human soluble CD163 (sCD163) in biological samples including serum, plasma, tissue homogenates, and other biological fluids. This kit provides high sensitivity, excellent specificity, and reproducible results for immunology and inflammatory disease research.

According to the product specification (Page 1), the kit offers high sensitivity of 4.5 pg/mL and a detection range of 15.63–1000 pg/mL, ensuring accurate detection even at low concentrations.

Key Features

• High sensitivity: 4.5 pg/mL
• Detection range: 15.63–1000 pg/mL
• Sandwich ELISA format
• High specificity with minimal cross-reactivity
• Reliable and reproducible results
• 96-well plate format
• Colorimetric detection at 450 nm
• Research use only

Intended Use

The Human sCD163 ELISA Kit is designed for quantitative detection of Human sCD163 in:

• Serum
• Plasma
• Tissue homogenates
• Cell lysates
• Urine
• Saliva
• Feces
• Cerebrospinal fluid
• Cell culture supernatant
• Other biological fluids

According to Sample Collection Guidelines (Page 4–5), the kit supports a wide range of biological sample types for flexible research applications.

Assay Principle

According to the Test Principle (Page 3), this kit uses sandwich ELISA technology:

1. Pre-coated anti-human sCD163 antibody binds target protein
2. Biotin-conjugated detection antibody binds sCD163
3. Streptavidin-HRP is added
4. TMB substrate produces color
5. Stop solution terminates reaction
6. Absorbance measured at 450 nm

The color intensity is proportional to the concentration of sCD163 in the sample.

Kit Components

Based on Kit Components Table (Page 1), the kit includes:

• Pre-coated ELISA microplate
• Lyophilized standard
• Biotinylated antibody
• Streptavidin-HRP
• Sample dilution buffer
• Antibody diluent
• HRP diluent
• Wash buffer (25×)
• TMB substrate solution
• Stop reagent
• Plate covers

Performance Characteristics

Parameter	Specification
Assay Type	Sandwich ELISA
Detection Range	15.63–1000 pg/mL
Sensitivity	4.5 pg/mL
Detection Method	Colorimetric
Sample Types	Serum, Plasma, Tissue, Fluids
Assay Format	96 Tests
Detection Wavelength	450 nm

Precision and Accuracy

According to Precision Data (Page 12):

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

Recovery performance:

• Serum: 92–105%
• EDTA plasma: 90–105%
• Heparin plasma: 85–97%

Linearity

The kit demonstrates strong linearity across dilution ranges:

• 1:2 dilution: 95–102%
• 1:4 dilution: 89–96%
• 1:8 dilution: 87–98%
• 1:16 dilution: 85–93%

Assay Procedure Overview

According to Assay Summary (Page 6):

1. Add standards and samples
2. Incubate at 37°C
3. Add biotinylated antibody
4. Add Streptavidin-HRP
5. Add TMB substrate
6. Add stop solution
7. Measure absorbance at 450 nm

Applications

• Immunology research
• Inflammation research
• Macrophage activation studies
• Biomarker discovery
• Autoimmune disease research
• Clinical research
• Molecular biology research

Storage Conditions

• Store at 4°C or -20°C
• Avoid repeated freeze-thaw cycles
• Store components properly after opening
• Follow storage instructions for optimal performance

Technical Specifications

• Product Name: Human sCD163 ELISA Kit
• Catalog Number: ELK8938
• Species: Human
• Assay Type: Sandwich ELISA
• Format: 96 Tests
• Detection Method: Colorimetric
• Sensitivity: 4.5 pg/mL
• Detection Range: 15.63–1000 pg/mL

Research Use Statement

This product is intended for research use only and not for diagnostic or therapeutic applications.