Human DPD (Deoxypyridinoline) ELISA Kit (E-EL-H2020 96T )
The Human DPD (Deoxypyridinoline) ELISA Kit (E-EL-H2020) is designed for the quantitative determination of Human DPD in serum, plasma, and other biological fluids using a Competitive ELISA method. This high-sensitivity assay offers a detection range of 3.125–200 ng/mL with excellent repeatability and specificity. Ideal for research laboratories, academic institutions, and biotechnology applications.
Key Features:
- Detection Range: 3.125–200 ng/mL
- Sensitivity: 1.875 ng/mL
- Sample Type: Serum, Plasma, Biological Fluids
- Assay Method: Competitive ELISA
- Format: 96 Tests
- High specificity and repeatability (<10% CV)
- For Research Use Only
Description
Human DPD (Deoxypyridinoline) ELISA Kit – E-EL-H2020 (96 Tests)
The Human DPD (Deoxypyridinoline) ELISA Kit is a high-performance immunoassay designed for quantitative detection of Human DPD concentrations in serum, plasma, and other biological fluids. This kit employs a Competitive ELISA methodology ensuring reliable and reproducible results for research applications.
This assay provides excellent sensitivity, specificity, and reproducibility, making it suitable for biomedical research, bone metabolism studies, and biomarker analysis.
Key Features
- High Sensitivity: 1.875 ng/mL
- Detection Range: 3.125–200 ng/mL
- High Specificity for Human DPD
- Competitive ELISA Method
- Excellent Repeatability (CV <10%)
- 96-Well Plate Format
- Research Use Only
The assay recognizes both natural and recombinant Human DPD with minimal cross-reactivity, ensuring high analytical accuracy.
Principle of the Assay
This kit uses a Competitive ELISA technique where:
- Microplate wells are pre-coated with DPD antigen
- Sample DPD competes with plate-bound DPD
- Biotinylated detection antibody binds to DPD
- HRP conjugate reacts with substrate
- Color intensity measured at 450 nm
- DPD concentration calculated using standard curve
This methodology ensures high sensitivity and accurate quantification.
Kit Components
The kit includes all necessary reagents for performing the assay:
- Pre-coated Micro ELISA Plate (96 wells)
- Reference Standards
- Sample Diluent
- Biotinylated Detection Antibody
- HRP Conjugate
- Wash Buffer (25×)
- Substrate Reagent
- Stop Solution
- Plate Sealers
- Instruction Manual
- Certificate of Analysis
Sample Types
This kit supports multiple biological samples:
- Serum
- Plasma
- Cell Culture Supernatant
- Tissue Homogenates
- Other Biological Fluids
Performance Characteristics
| Parameter | Specification |
| Detection Method | Competitive ELISA |
| Detection Range | 3.125–200 ng/mL |
| Sensitivity | 1.875 ng/mL |
| Sample Type | Serum, Plasma, Biological Fluids |
| Assay Time | Approx. 2–3 hours |
| Plate Format | 96 Wells |
| Storage | 2–8°C |
Applications
- Bone metabolism research
- Osteoporosis studies
- Biomarker analysis
- Clinical research applications
- Pharmaceutical research
- Academic laboratory studies
Storage & Stability
- Store all reagents at 2–8°C
- Avoid repeated freeze-thaw cycles
- Protect substrate from light
- Shelf life: 6 months under recommended storage conditions
Assay Summary
- Add standards and samples
- Add detection antibody
- Incubate at 37°C
- Wash plate
- Add HRP conjugate
- Add substrate
- Stop reaction
- Read absorbance at 450 nm
- Calculate results using standard curve
Intended Use
This kit is designed for research use only and is not intended for clinical diagnostic procedures.
Ordering Information
- Product Name: Human DPD (Deoxypyridinoline) ELISA Kit
- Catalog No: E-EL-H2020
- Assay Format: 96 Tests
- Species: Human
- Method: Competitive ELISA
