Description

Rabbit IL1b (Interleukin-1 Beta) ELISA Kit – ELK1228

The Rabbit IL1b (Interleukin-1 Beta) ELISA Kit (Cat. No. ELK1228) is a high-performance immunoassay designed for the quantitative measurement of IL-1β levels in rabbit biological samples. Interleukin-1 beta is a key pro-inflammatory cytokine involved in immune regulation, inflammatory responses, and cellular signaling processes.

This ELISA kit employs a sandwich enzyme immunoassay technique that enables accurate and reproducible quantification of Rabbit IL1β across a broad dynamic range. The kit is optimized for research laboratories studying inflammatory pathways, immune signaling, and cytokine regulation in rabbit models.

Key Features

High Sensitivity Detection

The assay provides high analytical sensitivity with a minimum detectable concentration of:

3 pg/mL

This allows accurate detection of low levels of IL1β in biological samples.

Wide Detection Range

The kit quantifies Rabbit IL1β across the following dynamic range:

7.82 pg/mL – 500 pg/mL

This range supports detection across various physiological and experimental conditions.

Excellent Specificity

The assay uses highly specific antibodies against Rabbit IL1β and demonstrates minimal cross-reactivity with related cytokines, ensuring reliable and accurate measurements.

High Precision and Reproducibility

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

These parameters ensure consistent results across different assays and experimental runs.

Assay Principle

The Rabbit IL1b ELISA Kit is based on the sandwich ELISA method:

1. Microplate wells are pre-coated with antibodies specific to Rabbit IL1β.
2. Standards and samples are added and bind to the immobilized antibodies.
3. A biotin-conjugated detection antibody specific to Rabbit IL1β is introduced.
4. Streptavidin-HRP binds to the antibody complex.
5. Addition of TMB substrate generates a colorimetric reaction proportional to the IL1β concentration.
6. The reaction is stopped and absorbance is measured at 450 nm.

The intensity of the color signal correlates directly with the concentration of Rabbit IL1β present in the sample and is calculated using a standard curve.

Compatible Sample Types

This ELISA kit supports detection of Rabbit IL1β in multiple biological samples including:

• Serum
• Plasma
• Tissue Homogenates
• Cell Lysates
• Cell Culture Supernatants
• Other Biological Fluids

Proper sample preparation and storage are recommended to preserve analyte stability and ensure accurate results.

Kit Components

The Rabbit IL1b ELISA Kit contains all essential reagents required for assay performance:

• Pre-Coated ELISA Microplate
• Lyophilized Rabbit IL1β Standard
• Biotinylated Detection Antibody
• Streptavidin-HRP Conjugate
• Standard/Sample Dilution Buffer
• Biotinylated Antibody Diluent
• HRP Diluent
• Wash Buffer (25×)
• TMB Substrate Solution
• Stop Reagent
• Plate Covers

All components are quality-tested and optimized to ensure reliable experimental outcomes.

Applications

The Rabbit IL1b ELISA Kit is widely used in research fields such as:

• Cytokine and inflammation research
• Immunology studies
• Infection and immune response studies
• Drug discovery and pharmacological research
• Animal model studies involving inflammatory signaling pathways

Storage Conditions

To maintain optimal assay performance:

• Short-term storage: 4°C (up to 6 months)
• Long-term storage: −20°C (up to 1 year)
• Avoid repeated freeze-thaw cycles.

Important Notice

For Research Use Only (RUO).
This product is intended solely for laboratory research applications and not for diagnostic or therapeutic use.