Human Interleukin 8, IL-8 ELISA Kit E0089Hu 96T
The Human Interleukin-8 (IL-8) ELISA Kit (E0089Hu) is a high-sensitivity sandwich ELISA designed for the quantitative detection of Human IL-8 in serum, plasma, cell culture supernatants, tissue homogenates, and other biological fluids. With a detection range of 5–1000 ng/L and sensitivity of 2.51 ng/L, this kit ensures accurate and reproducible research results.
Key Features:
- Detection Range: 5–1000 ng/L
- Sensitivity: 2.51 ng/L
- Assay Type: Sandwich ELISA
- Sample Type: Serum, Plasma, Cell Culture Supernatant, Tissue, Biological Fluids
- Format: 96 Tests
- High Precision (CV <10%)
Research Use Only
Description
Human Interleukin-8 (IL-8) ELISA Kit (E0089Hu) – 96 Tests
The Human Interleukin-8 (IL-8) ELISA Kit is a high-performance sandwich ELISA assay developed for the quantitative detection of Human IL-8 in various biological samples including serum, plasma, cell culture supernatants, tissue homogenates, ascites, and other biological fluids.
This kit delivers reliable and reproducible results with high sensitivity and precision, making it ideal for research laboratories, clinical research, biotechnology, and academic institutions.
Key Features
- High Sensitivity: 2.51 ng/L
- Detection Range: 5–1000 ng/L
- Sandwich ELISA Method
- High Precision (CV <10%)
- 96-Well Plate Format
- Multiple Sample Compatibility
- Research Use Only
The assay demonstrates excellent intra-assay and inter-assay precision, ensuring consistent results across experiments.
Intended Use
This ELISA kit is designed for quantitative measurement of Human Interleukin-8 (IL-8) in:
- Serum
- Plasma
- Cell Culture Supernatants
- Tissue Homogenates
- Ascites
- Other Biological Fluids
Assay Principle
This kit utilizes a Sandwich ELISA technique:
- Microplate wells are pre-coated with Human IL-8 antibody
- Samples containing IL-8 bind to coated antibodies
- Biotinylated IL-8 antibody is added
- Streptavidin-HRP binds to antibody complex
- Substrate reaction produces measurable color
- Reaction stopped and absorbance measured at 450 nm
Color intensity is proportional to IL-8 concentration in the sample.
Kit Components
The kit includes:
- Pre-coated ELISA Plate (96 wells)
- Standard Solution
- Standard Diluent
- Biotinylated Human IL-8 Antibody
- Streptavidin-HRP
- Wash Buffer Concentrate (25×)
- Substrate Solution A
- Substrate Solution B
- Stop Solution
- Plate Sealers
- User Manual
Performance Characteristics
| Parameter | Specification |
| Assay Type | Sandwich ELISA |
| Detection Range | 5–1000 ng/L |
| Sensitivity | 2.51 ng/L |
| Sample Type | Serum, Plasma, Tissue, Cell Culture |
| Assay Time | Approx. 90–120 minutes |
| Plate Format | 96 Wells |
| Storage | -20°C or 2–8°C |
Sample Types Supported
- Serum
- Plasma
- Cell Culture Supernatant
- Tissue Homogenates
- Urine
- Ascites
- Cerebrospinal Fluid
Applications
- Cytokine Research
- Inflammation Studies
- Immunology Research
- Cancer Research
- Drug Discovery
- Biomedical Research
- Academic Research
Storage and Stability
- Store at -20°C for 12 months
- Store at 2-8°C for 6 months
- Use opened reagents within 1 month
- Avoid repeated freeze-thaw cycles
Assay Summary
- Add standards and samples
- Add antibody and HRP reagent
- Incubate at 37°C
- Wash plate
- Add substrate solutions
- Stop reaction
- Measure absorbance at 450 nm
- Calculate results using standard curve
Ordering Information
- Product Name: Human Interleukin-8 (IL-8) ELISA Kit
- Catalog Number: E0089Hu
- Assay Type: Sandwich ELISA
- Species: Human
- Format: 96 Tests
Research Use Statement
This product is intended for research use only and not for diagnostic or therapeutic applications.
