Human CGRP1(Calcitonin Gene Related Peptide 1) ELISA Kit ELK1209 96T
The ELK Biotechnology Human CGRP1 ELISA Kit is a high-sensitivity, sandwich-mode enzyme immunoassay designed for the precise quantitative measurement of Calcitonin Gene Related Peptide 1 in human biological fluids. This research-grade kit offers an excellent detection range of 15.63–1000 pg/mL with a minimal sensitivity of 4.52 pg/mL, ensuring reliable results even in samples with low analyte concentrations.
Key Features:
- High Specificity: Optimized to detect Human CGRP1 with no significant cross-reactivity or interference from structural analogues.
- Versatile Sample Compatibility: Validated for use with serum, plasma (EDTA/Heparin), tissue homogenates, cell lysates, and cell culture supernatants.
- Rapid Procedure: Streamlined 3.5-hour protocol with clear, step-by-step incubation and wash cycles.
- Excellent Precision: Features high reproducibility with Intra-assay CV <8% and Inter-assay CV <10%.
- Robust Shelf Life: Stable for up to 6 months at $4^{\circ}C$ or 1 year at $-20^{\circ}C$ when unopened.
Description
Overview:
The Human CGRP1 (Calcitonin Gene Related Peptide 1) ELISA Kit is a specialized tool for researchers investigating neuroendocrine signaling, cardiovascular regulation, and pain pathways. Utilizing the Sandwich ELISA principle, this kit provides a quantitative “gold standard” approach for measuring Human CGRP1 levels across a variety of complex matrices.
Principle of the Assay
This assay employs a pre-coated microtiter plate with an antibody specific to Human CGRP1. Standards or samples are added to the wells, followed by a biotin-conjugated antibody specific to the target. Subsequent addition of Avidin-conjugated Horseradish Peroxidase (HRP) creates an immune complex. After the addition of TMB substrate, the color change is directly proportional to the amount of CGRP1 present, which is then measured spectrophotometrically at 450 nm.
Technical Specifications
- Sensitivity: $4.52~pg/mL$
- Detection Range: $15.63-1000~pg/mL$
- Assay Time: Approximately 3.5 hours (excluding reagent preparation)
- Recovery Rates: High accuracy across matrices, including Serum (86%), EDTA Plasma (98%), and Heparin Plasma (95%).
Kit Components (96T)
Everything required for the assay is provided in a convenient format:
- Pre-Coated Microplate (12 strips x 8 wells)
- Lyophilized Standard (2 vials)
- Biotinylated Antibody (100x) & Streptavidin-HRP (100x)
- Standard/Sample Diluent, Antibody Diluent, and HRP Diluent
- TMB Substrate & Stop Solution
- 25x Wash Buffer
Storage and Stability
For long-term preservation, the unopened kit is valid for 1 year when stored at $-20^{\circ}C$. Once opened, the kit components are stable for up to 1 week at $4^{\circ}C$ or 6 months if critical components (Plate, Standard, Antibodies) are stored at $-20^{\circ}C$.
Usage Notes
- Research Use Only: This product is not intended for diagnostic or clinical procedures.
- Sample Preparation: Avoid using hemolyzed samples or those containing sodium azide (NaN3), as these can interfere with HRP activity.
