Description

Human SDMA (Symmetric Dimethylarginine) ELISA Kit – ELK9785

The Human Symmetric Dimethylarginine (SDMA) ELISA Kit (Cat. No. ELK9785) is a highly sensitive immunoassay designed for the quantitative determination of SDMA concentrations in human biological samples. SDMA is a methylated derivative of arginine and is widely studied as a biomarker associated with renal function, nitric oxide metabolism, and cardiovascular health.

This kit employs a competitive inhibition enzyme immunoassay to measure SDMA levels accurately in serum and EDTA plasma samples, making it suitable for a variety of biomedical and clinical research applications.

Key Features

High Sensitivity Detection

The assay provides excellent analytical sensitivity with a minimum detectable concentration of:

0.12 nmol/mL

This allows researchers to detect low concentrations of SDMA in biological samples.

Wide Detection Range

The kit provides accurate quantification across the following concentration range:

0.32 nmol/mL – 20 nmol/mL

This broad dynamic range supports reliable detection across multiple experimental conditions.

Excellent Specificity

The assay uses highly specific antibodies to detect Human SDMA and demonstrates minimal cross-reactivity with similar molecules or analogues, ensuring reliable analytical performance.

Reliable Precision

Performance evaluation demonstrates strong reproducibility:

• Intra-assay precision: CV < 8%
• Inter-assay precision: CV < 10%

These parameters ensure consistent assay performance across multiple runs.

Assay Principle

The Human SDMA ELISA Kit utilizes a competitive inhibition enzyme immunoassay technique:

1. Microplate wells are pre-coated with SDMA antigen.
2. Standards or samples are added together with a biotinylated SDMA conjugate.
3. The SDMA present in the sample competes with the coated antigen for antibody binding.
4. Streptavidin-HRP is then added to bind with the biotinylated complex.
5. Addition of TMB substrate results in a colorimetric reaction.
6. The reaction is stopped and absorbance is measured at 450 nm.

Because the assay is competitive, the color intensity is inversely proportional to the SDMA concentration in the sample.

Compatible Sample Types

This ELISA kit is optimized for detection of SDMA in:

• Human Serum
• EDTA Plasma

Samples should be properly collected, centrifuged, and stored at −20°C or −80°C to maintain analyte stability and prevent degradation.

Kit Components

The Human SDMA ELISA Kit contains all necessary reagents for assay performance:

• Pre-Coated ELISA Microplate
• Lyophilized SDMA Standard
• Biotinylated Conjugate
• Streptavidin-HRP Conjugate
• Standard/Sample Dilution Buffer
• Biotinylated-Conjugate Diluent
• HRP Diluent
• Wash Buffer (25×)
• TMB Substrate Solution
• Stop Reagent
• Plate Covers

Each component is optimized and quality tested to ensure reliable experimental results.

Applications

This ELISA kit is suitable for a wide range of biomedical research applications, including:

• Kidney function and renal disease research
• Nitric oxide metabolism studies
• Cardiovascular research
• Biomarker discovery
• Drug development and pharmacological studies

Storage Conditions

To maintain optimal performance:

• Short-term storage: 4°C (up to 6 months)
• Long-term storage: −20°C (up to 1 year)
• Avoid repeated freeze–thaw cycles to preserve reagent stability.

Important Notice

For Research Use Only (RUO).
This product is intended strictly for laboratory research and not for diagnostic or therapeutic use.