RNeasy® Mini Kit 74106
RNeasy® Mini Kit (74106) is a reliable silica-membrane spin column kit designed for rapid purification of high-quality total RNA from animal cells and tissues. The kit ensures efficient RNA isolation with minimal contamination, making it ideal for gene expression, RT-PCR, and molecular biology applications.
Key Features
- Rapid purification of high-quality total RNA
- Silica-membrane spin column technology
- Suitable for cells and tissue samples
- Process up to 1 × 10⁷ cells or 30 mg tissue
- Fast workflow (~20 minutes)
- High RNA purity and yield
- DNase digestion option available
- Ready-to-use buffers included
- Room temperature storage (15–25°C)
- Ideal for RT-PCR, qPCR, NGS, and gene expression analysis
Description
RNeasy® Mini Kit (74106)
The RNeasy® Mini Kit (74106) provides a fast and efficient method for purification of high-quality total RNA from animal cells and tissues. Using advanced silica-membrane spin column technology, this kit delivers consistent RNA yield and purity suitable for downstream molecular biology applications.
This kit is designed for laboratories requiring rapid RNA isolation with minimal handling steps and reliable performance.
Product Overview
- Product Name: RNeasy® Mini Kit
- Catalog Number: 74106
- Technology: Silica-membrane spin column
- Sample Types: Animal cells and tissues
- Storage: Room temperature (15–25°C)
- Application: RNA purification and gene expression analysis
Sample Compatibility
The RNeasy Mini Kit supports purification from:
- Animal cells
- Tissue samples
- Cultured cells
- Homogenized tissue samples
- Up to 1 × 10⁷ animal cells
- Up to 30 mg tissue sample
Key Benefits
High-Quality RNA Purification
The kit utilizes silica membrane technology to isolate RNA efficiently while removing contaminants such as proteins, DNA, and enzymatic inhibitors.
Fast Workflow
The protocol provides rapid RNA purification in a simple workflow involving:
- Sample lysis and homogenization
- Ethanol addition
- Spin column binding
- Wash steps
- RNA elution
Assay Workflow Overview
- Lyse cells or tissues using Buffer RLT
- Add ethanol to lysate
- Transfer to spin column
- Wash using Buffer RW1
- Wash with Buffer RPE
- Elute RNA using RNase-free water
The RNA is then ready for downstream applications.
Applications
The RNeasy Mini Kit is suitable for:
- RT-PCR
- qPCR
- Gene expression analysis
- RNA sequencing
- Microarray analysis
- Northern blotting
- Molecular biology research
Performance Features
- High RNA yield
- High purity RNA
- Minimal genomic DNA contamination
- Fast processing time
- Reliable reproducibility
- Easy-to-follow protocol
Optional DNase Digestion
The kit supports on-column DNase digestion to remove genomic DNA contamination, ensuring high-quality RNA for sensitive downstream applications.
Storage and Stability
- Store at room temperature (15–25°C)
- Stable for at least 9 months when stored properly
- Follow buffer preparation instructions before use
Workflow Summary
| Step | Description |
| 1 | Sample lysis |
| 2 | Ethanol addition |
| 3 | Column binding |
| 4 | Wash RW1 |
| 5 | Wash RPE |
| 6 | RNA Elution |
Technical Specifications
| Parameter | Details |
| Product Name | RNeasy® Mini Kit |
| Catalog No | 74106 |
| Sample Type | Cells, Tissue |
| Max Cell Input | 1 × 10⁷ cells |
| Max Tissue Input | 30 mg |
| Storage | Room temperature |
| Technology | Spin Column |
Advantages
- Rapid RNA purification
- High purity RNA isolation
- Minimal sample handling
- Consistent performance
- Compatible with multiple downstream applications
The RNeasy® Mini Kit (74106) provides researchers with a reliable and efficient RNA purification solution, making it ideal for gene expression studies, molecular biology workflows, and RNA-based research applications.
