Trypsin-EDTA (0.25%), phenol red 25200-072
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Trypsin-EDTA (0.25%), phenol red 25200-072

Trypsin-EDTA (0.25%), Phenol Red (25200-072) is a high-quality cell dissociation reagent designed for detachment of adherent cells in tissue culture applications. This sterile, ready-to-use solution provides consistent performance, optimal pH range, and reliable cell harvesting for research laboratories.

Key Features

  • Product: Trypsin-EDTA (0.25%), Phenol Red
  • Product Number: 25200-072
  • Format: 1X Liquid Solution
  • Trypsin Concentration: 0.25%
  • Sterile and ready-to-use formulation
  • pH Range: 7.2 – 8.0
  • Osmolality: 270 – 320 mOsm/kg
  • Mycoplasma tested (Negative)
  • Sterility tested (Negative)
  • Porcine Parvovirus tested (Negative)
  • Storage Temperature: -5°C to -20°C
  • Research Use Only (RUO)
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Description

Trypsin-EDTA (0.25%), Phenol Red – 25200-072

Trypsin-EDTA (0.25%), Phenol Red (25200-072) is a high-quality cell dissociation reagent designed for efficient detachment of adherent cells in tissue culture applications. This ready-to-use sterile solution ensures consistent performance and reliable cell harvesting across research laboratories.

This reagent combines 0.25% Trypsin with EDTA to enhance cell dissociation efficiency while maintaining cell viability. The inclusion of phenol red allows visual monitoring of pH conditions during cell culture procedures.

Product Overview

  • Product Name: Trypsin-EDTA (0.25%), Phenol Red
  • Product Number: 25200-072
  • Format: 1X Liquid
  • Trypsin Concentration: 0.25%
  • Storage Temperature: -5°C to -20°C
  • Application: Cell dissociation reagent
  • Use: Research Use Only

Product Features

  • Ready-to-use sterile solution
  • Optimized for cell culture applications
  • Reliable cell detachment performance
  • Includes phenol red for pH monitoring
  • Consistent quality and performance
  • Tested for sterility and contaminants

Quality Control Specifications

According to the Certificate of Analysis:

Parameter Specification Result
In-Vitro Bioassay Acceptable Acceptable
Mycoplasma Testing Negative Negative
Osmolality 270–320 mOsm/kg 288 mOsm/kg
pH 7.2–8.0 7.8
Porcine Parvovirus Negative Negative
Sterility Negative Negative

These results confirm reliable performance and laboratory-grade quality.

Applications

Trypsin-EDTA is commonly used for:

  • Cell dissociation
  • Tissue culture applications
  • Adherent cell detachment
  • Cell passaging
  • Cell harvesting
  • Laboratory cell culture workflows

Storage and Stability

  • Storage Temperature: -5°C to -20°C
  • Maintain sterile conditions after opening
  • Avoid repeated freeze-thaw cycles
  • Use according to laboratory protocols

Quality Assurance

  • Mycoplasma tested
  • Virus tested
  • Sterility tested
  • Bioassay verified

Raw stock trypsin is verified for e-beam irradiation and tested negative for contamination sources ensuring safe laboratory usage.

Technical Specifications

Parameter Details
Product Name Trypsin-EDTA
Concentration 0.25%
Format 1X Liquid
pH Range 7.2 – 8.0
Osmolality 270 – 320 mOsm/kg
Storage -5°C to -20°C

Advantages

  • Reliable cell detachment
  • High quality formulation
  • Sterile and ready to use
  • Consistent performance
  • Quality tested

Trypsin-EDTA (0.25%), Phenol Red – 25200-072 provides researchers with a reliable, sterile, and efficient solution for cell culture and tissue dissociation applications, making it an essential reagent for life science laboratories.

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