QIAamp Viral RNA Mini Kit (50) 52904
QIAamp Viral RNA Mini Kit (50) – 52904 is designed for rapid and reliable purification of viral RNA from plasma, serum, and cell-free body fluids. Using silica membrane technology, this kit provides high-quality viral RNA suitable for RT-PCR, PCR, and molecular biology applications. The kit ensures fast processing, high sensitivity, and reproducible results.
Key Features
- Purifies viral RNA from plasma, serum, and cell-free fluids
- High-quality RNA suitable for RT-PCR and PCR
- Silica membrane spin column technology
- Fast and efficient purification workflow
- Carrier RNA included for improved sensitivity
- Compatible with manual and automated workflows
- Minimal cross-contamination risk
- Room temperature storage for convenience
- 50 preparations per kit
Catalog No: 52904
Description
QIAamp Viral RNA Mini Kit (50) – 52904
The QIAamp Viral RNA Mini Kit (50) – 52904 is a high-performance RNA purification kit designed for isolation of viral RNA from plasma, serum, and cell-free body fluids. The kit combines silica membrane technology with optimized buffers to deliver rapid and reliable purification suitable for downstream molecular biology applications.
This kit provides a fast, efficient, and reproducible method for purification of viral RNA for use in RT-PCR, PCR, sequencing, and other molecular biology applications.
Intended Use
The QIAamp Viral RNA Mini Kit is designed for purification of viral RNA from:
- Plasma
- Serum
- Cell-free body fluids
- Culture supernatants
The purified RNA is suitable for molecular biology applications and downstream amplification technologies. The kit is intended for research use and not for diagnostic, prevention, or treatment purposes.
Principle of the Assay
The kit uses silica-based membrane technology combined with spin-column or vacuum processing:
- Sample lysis using Buffer AVL
- Binding of viral RNA to silica membrane
- Washing to remove contaminants
- Elution of purified viral RNA
This workflow ensures high-quality RNA isolation and minimal contamination.
Kit Contents (50 Preparations)
The QIAamp Viral RNA Mini Kit (50) includes:
- 50 QIAamp Mini Spin Columns
- 150 Collection Tubes (2 mL)
- Buffer AVL (31 mL)
- Buffer AW1 (Concentrate)
- Buffer AW2 (Concentrate)
- Buffer AVE
- Carrier RNA (poly A)
Key Features and Benefits
High Sensitivity
Carrier RNA improves binding efficiency and enhances RNA recovery, particularly from low viral titer samples.
Fast and Efficient Protocol
The purification procedure uses spin-column technology allowing rapid processing of multiple samples simultaneously.
Flexible Workflow
Compatible with:
- Spin protocol
- Vacuum protocol
- QIAcube automation
This provides flexibility in laboratory workflows.
Sample Types Supported
The kit supports purification of viral RNA from:
- Plasma
- Serum
- Urine
- Cell culture media
- Cell-free body fluids
Optimized for 140 µL sample volume, with flexibility for larger volumes.
Applications
- RT-PCR
- Real-time PCR
- Viral RNA detection
- Molecular diagnostics research
- Virology studies
- Gene expression analysis
- Sequencing applications
Performance and Reliability
- High RNA purity
- Excellent reproducibility
- Minimal contamination risk
- Reliable downstream performance
- Efficient recovery of RNA >200 nucleotides
Storage and Stability
- Store spin columns at 15–25°C
- Buffers stored at room temperature
- Stable until expiration date
- No performance reduction under recommended storage conditions
Advantages
- Fast purification workflow
- High sensitivity detection
- Reliable results
- Flexible processing options
- Automated compatibility
- Minimal hands-on time
- Optimized buffer chemistry
Product Specifications
| Parameter | Details |
| Product Name | QIAamp Viral RNA Mini Kit |
| Catalog Number | 52904 |
| Preparations | 50 |
| Technology | Silica Membrane Spin Column |
| Sample Type | Plasma, Serum, Cell-Free Fluids |
| Applications | RT-PCR, PCR, Molecular Biology |
| Storage | Room Temperature |
The QIAamp Viral RNA Mini Kit (50) – 52904 offers a reliable, efficient, and high-quality solution for viral RNA purification, making it an essential tool for molecular biology, virology, and nucleic acid research laboratories.
