Description

CELLBANKER® 2 is a premium-grade, serum-free cryopreservation medium formulated to provide superior protection for cells during freezing and thawing processes. Designed for research applications, it simplifies cryopreservation workflows while maintaining high cell viability and integrity.

This ready-to-use solution eliminates the need for serum supplementation and supports consistent performance across various cell types, making it an essential reagent for modern cell culture laboratories.

Key Features & Benefits

• Serum-Free Formulation – Reduces variability and contamination risks associated with serum-based media
• High Cell Viability – Ensures ≥80% post-thaw viability across multiple cell lines
• Direct Freezing Capability – Cells can be directly stored at -80°C without controlled-rate freezing
• Wide Cell Compatibility – Suitable for mammalian cells including hybridoma, fibroblasts, tumor cells, and stem cells
• Quality Assured – Tested free from bacteria, fungi, and mycoplasma
• Stable & Reliable – Shelf life of up to 3 years under recommended storage conditions

Technical Specifications

• Product Name: CELLBANKER® 2
• Type: Cryopreservation Medium (Serum-Free)
• Catalog Number: 11914 (formerly 11891)
• Volume: 100 ml
• pH Range: 7.0 – 8.5
• Endotoxin Level: <5 EU/mL
• Application: Cell freezing and long-term storage
• Storage Condition: 4°C or below (freezable for long-term storage)

Applications

CELLBANKER® 2 is ideal for:

• Cryopreservation of mammalian cell lines
• Stem cell research
• Hybridoma and myeloma cell storage
• Cancer and primary cell research
• Biopharmaceutical and academic laboratories

Experimental data (pages 3–4) demonstrate consistent viability (~90–95%) across multiple species including mouse, rat, hamster, monkey, and human cells.

Protocol Overview

Cell Freezing Process

1. Ensure healthy, contamination-free cells in log phase
2. Centrifuge cells (3–5 min at 1000–2000 rpm, 4°C)
3. Remove supernatant and resuspend in CELLBANKER® 2
4. Dispense into cryovials (1 ml aliquots)
5. Store directly at -80°C, then transfer to liquid nitrogen if required

Thawing Process

1. Rapidly thaw cells in a 37°C water bath
2. Dilute with culture medium
3. Centrifuge and resuspend in fresh medium
4. Culture using standard protocols

Quality Assurance

• Sterility: Free from bacteria, fungi, and mycoplasma
• Performance tested for high viability
• Consistent reproducibility across experiments

Storage & Handling

• Store at 4°C or below
• Avoid repeated freeze-thaw cycles
• Aliquot before freezing for long-term use

Precautions

• For research use only
• Not intended for clinical or diagnostic applications
• Perform validation tests with your specific cell type before use

Why Choose CELLBANKER® 2?

CELLBANKER® 2 delivers a streamlined, reliable, and high-efficiency cryopreservation solution tailored for advanced research environments. Its serum-free composition, robust performance, and simplified workflow make it a preferred choice for laboratories aiming to preserve cell integrity with minimal complexity.